Sequences from confirmation amplicon


Confirmation sequences for line 639G03 and hit At5g65060 are shown.
Line ID 639G03
Locus At5g65060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25987344
Primers used for PCR KF27 - 8474, show primer details
   
Sequence Name 59-K103224-0022-639-G03C-KF27
GenBank Accession LN509970 [GenBank]
Sequencing primer gene specific primer, KF27: 5'-ATTAGGTTTGTGGTAACGCATAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K103224-0022-639-G03C-KF27
TCTCAGTTGATTTGAAGGAAGCAAAAAGCCCTAGATTTGAAGTTATTATATAAGATTAAA
AAAAGAAGAAGCACAAAACTCGAGTGAGACCCAGAAGAGGAAAGAAGCTTACTTGTCACC
GGAGGCAGAGTCGTAGAGTTTTCCGGAACCGGAGACGGCGACNACAGCGATGGAAGATTC
ACAGAGAATTGAAAGTTGTCGAGCTTTTTCGATGAGACCTTTGCGTCGTTTGGAGAAAGT
GACTTGTCGACTGCTTTTGTTCTCGATTCGCTTGATCTCGACTTTTCTTCTTCCCATGGC
TTCTTTCTCCGATAAGGTTTGTGCCCTAATTTGATCCTAACCACCGGGGACCCAAAATGT
GTTTGCTTTTTTTCTTCGTTTCTATCTTCTTCTTTTGGGCTAGACAAATGAAAAAAAATC
CCAAGTCAGTTTTTTTTTTTGTTAGACAATGGAGAGAAAAATAATAACAGGTGGAAACGG
CTTCTTGTCCGGGAAATCTACTGGGATCACCATTGAGTATGAGGGTCAATATGGAGAAAA
AGAAAGAGTATTTCCCATTTTTTTTTCATTTCAAAAATGTAAATTTCCCCC
   
Sequence Name 27-K103225-0022-639-G03C-8409
GenBank Accession LN498343 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K103225-0022-639-G03C-8409
GAGCCGTTTCCCTTGTTATTATTTTTCTCTCTATCGTCTAAAAAAAAAAAAACTGACTTG
GGATTTTTTTTCATTTGTCTAGCCCAAAAAAAAAAAATAGAAACGAAAAAAAAAAGCAAA
CCCTTTTTGGGTCCCCGGTGGTTAGGATCAAATTAGGGCACAAACCTTATCGAAAAAAAA
ACCCTTGGGAAAAAAAAAAGTCAAAATCAAGCGAATCGAGAACAAAAGCAGTCAACAAGT
CACTTTCTCCAAACAACGCAAAGGTCTCATCAAAAAAGCTCGACAACTTTCATTTCTCTG
TGAATCTTCCATCGCTGTTGTCCCCTTCCCCGGTTCCGGAAAACTCTACAACTCTCCCTC
CGGTGACAAGTAACCTTCTTTCCCCTTCGGGGTCTCACTCGAGTTTTGTGCTTCTTCTTT
TTTTAACCTAATATAAAAACTTCAAATCTGGGGCTTTTTGCTTCCTTCAAATCAACTGAA
ATCTCCCCCTTTTTTTTCTGTGTGAATAGAGCCAATGCTTT

Last Updated on 06.12.2018 16:11