Sequences from confirmation amplicon


Confirmation sequences for line 642C12 and hit At2g40750 are shown.
Line ID 642C12
Locus At2g40750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17000684
Primers used for PCR IS63 - 8474, show primer details
   
Sequence Name 23-K025674-022-642-C12C-8409
GenBank Accession LN496409 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K025674-022-642-C12C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGTACTCATTGCTGGAGGAA
AACTGATCATCGTTGTCGATGAAACCAAATTGATGAGAAGTAGTACTGGTTTCACCACAA
TAGTAGTGATTTTGATGATCATCAAACATCATCGTCTCTTGCCAAACCAATGACAAGTCC
TCACCTGTCGAAGAGCCCTCATAATAATCTTTACTCTGATCACCATTGTTATTTTGTTCC
TCCTTAACCATGCCTGCGTCTATTGCTGTCACACTGCTGGTGTTGTTCTCTTGCTCTTGC
ACCATAGCATTGACATGGTTCTGAGCAGTGCTAGCAGCCAATGTCTTTTCCGAATCCATA
ATGATTTCTTGATCAAAAGGCTCGGTCTTCGCGTGCGTTTGGTCATTGGCAGTGCATGTG
TGGTAGCCAATGTATGTGATTTGGAACATCTCAGAATCTTGATCCTGTTTCTGAACTTGC
TTTGTTGCTTTGCATCCTTGCGTTGGCTTGTGTGTGCATCTAAAGTAACTTCTGCAAAAC
ATTAAAACGCATATTGATTTTAGGCCATTGCAAACTTCACATAGTGACTTGAAATATAAC
ATATGTTTCGGCTAGAGATCCCTGGTATAAAAATGAGTTGCCCATGTTTATATGTATTAT
ATATGTATTGTGAGTATATAGATACATAAATAGACATATAAGGAGTTAGGTAA
   
Sequence Name 24-K025674-022-642-C12C-IS63
GenBank Accession LN496849 [GenBank]
Sequencing primer gene specific primer, IS63: 5'-TTCTTAATACCACATTCCCAAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K025674-022-642-C12C-is63
TAATTACCTAACTCCTTATATGTCTATTTATGTATCTATATACTCACAATACATATATAA
TACATATAAACATGGGCAACTCATTTTTATACCAGGGATCTCTAGCCGAAACATATGTTA
TATTTCAAGTCACTATGTGAAGTTTGCAATGGCCTAAAATCAATATGCGTTTTAATGTTT
TGCAGAAGTTACTTTAGATGCACACACAAGCCAACGCAAGGATGCAAAGCAACAAAGCAA
GTTCAGAAACAGGATCAAGATTCTGAGATGTTCCAAATCACATACATTGGCTACCACACA
TGCACTGCCAATGACCAAACGCACGCGAAGACCGAGCCTTTTGATCAAGAAATCATTATG
GATTCGGAAAAGACATTGGCTGCTAGCACTGCTCAGAACCATGTCAATGCTATGGTGCAA
GAGCAAGAGAACAACACCAGCAGTGTGACAGCAATAGACGCAGGCATGGTTAAGGAGGAA
CAAAATAACAATGGTGATCAGAGTAAAGATTATTATGAGGGCTCTTCGACAGGTGAGGAC
TTGTCATTGGTTTGGCAAGAGACGATGATGTTTGATGATCATCAAAATCACTACTATTGT
GGTGAAACCAGTACTACTTCTCATCAATTTGGTTTCATCGACAACGATGATCAGTTTTCC
TCCAGCAATGAGTACCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTATTACCAATTTT
TTTTCAT

Last Updated on 06.12.2018 16:11