Sequences from confirmation amplicon


Confirmation sequences for line 642H10 and hit At2g24840 are shown.
Line ID 642H10
Locus At2g24840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10581244
Primers used for PCR KQ52 - 8474, show primer details
   
Sequence Name 51-K101565-0022-642-H10C-8409
GenBank Accession LN507449 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K101565-0022-642-H10C-8409
CCATTTACATTGAATATGTCGAAGAAGAAAGAAAGCATCGGACGACAAAAAATTCCAATG
GTAAAAATAAAGAAAGAGAGCCACCGGCAAGTCACATTCTCCAAACGCAGAGCCGGTCTC
TTCAAGAAAGCTAGTGAGCTTTGCACTTTGTGTGGTGCAGAGATTGGGATCATCGTGTTT
TCTCCCGCGAAAAAGCCTTTCTCATTCGGACATCCAAGTGTTGAATCTGTATTGGATCGC
TACGTGTCTCGAAACAATATGTCCTTAGCTCAGTCGCAGCAACTGCAAGGAAGCCCTGCA
GCGAGCTGCGAACTGAATATGCAGTTAACGCATATTTTGAGCGAGGTAGAAGAAGAGAAG
AAGAAGGGTCAAGCGATGGAAGAGATGAGAAAAGAGAGTGTGAGG
   
Sequence Name 51-K101578-0022-642-H10C-KQ52
GenBank Accession LN507450 [GenBank]
Sequencing primer gene specific primer, KQ52: 5'-GCCTCACACTCTCTTTTCTCATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K101578-0022-642-H10C-KQ52
CTCGCTCAAATATGCGTTAACTGCATATTCAGTTCGCAGCTCGCTGCAGGGCTTCCTTGC
AGTTGCTGCGACTGAGCTAAGGACATATTGTTTCGAGACACGTAGCGATCCAATACAGAT
TCAACACTTGGATGTCCGAATGAGAAAGGCTTTTTCGCGGGAGAAAACACGATGATCCCA
ATCTCTGCACCACACAAAGTGCAAAGCTCACTAGCTTTCTTGAAGAGACCGGCTCTGCGT
TTGGAGAATGTGACTTGCCGGTGGCTCTCTTTCTTTATTTTTACCATTGGAATTTTTTGT
CGTCCGATGCTTTCTTTCTTCTTCGACATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATNTCCGCA

Last Updated on 06.12.2018 16:11