Sequences from confirmation amplicon


Confirmation sequences for line 649D05 and hit At1g30810 are shown.
Line ID 649D05
Locus At1g30810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10938503
Primers used for PCR DE51 - 8474, show primer details
   
Sequence Name 58-K104987-0022-649-D05C-DE51
GenBank Accession LN509700 [GenBank]
Sequencing primer gene specific primer, DE51: 5'-TCTCGGAGTTGGTACAGTCTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K104987-0022-649-D05C-DE51
TTTGGTTAACTTGCGGAACAGTTTGATGATATGAAGTGATCTTTTGAGTCTGATGAAGTT
TGGTTCTTGTGGTTCCAGTACTCAACTAGTCGATGGTTTGGGTCAAGAGCTTCAATGGCC
TGTAGAGAAGCGTTAAGTTGTGTAAGAGTTAACATCATGAATAGCTTTTTAATGAAGAAG
AACAAGGCTTGGATTTAGATTTCTTACCTGAACTATAGAGGGTGAGAGGAAACCAAACAT
TTGAAGCCCGTTGATACTCTCAAACTGTGGTAAAATAGGAAGACCAAGACTTGTTGATGT
ATCTTTAACTCTCCGCATCACCATTTCCCAGCATTGTTGTGCAGAGACATTGAAGAAGCT
CTCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCC
   
Sequence Name 42-K104988-0022-649-D05C-8409
GenBank Accession LN504457 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K104988-0022-649-D05C-8409
CATGCTGGGAATGGTGATGCGGAGAGTTAAAGATACATCAACAAGTCTTGGTCTTCCTAT
TTTACCACAGTTTGAGAGTATCAACGGGCTTCAAATGTTTGGTTTCCTCTCACCCTCTAT
AGTTCAGGTAAGAAATCTAAATCCAAGCCTTGTTCTTCTTCATTAAAAAGCTATTCATGA
TGTTAACTCTTACACAACTTAACGCTTCTCTACAGGCCATTGAAGCTCTTGACCCAAACC
ATCGACTAGTTGAGTACTGGAACCACAAGAACCAAACTTCATCAGACTCAAAAGATCACT
TCATATCATCAAACTGTTCCGCAAGTTTAACCAAAGGAAAACTTTTCGGAGTAGATTTGA
TGTAAACAAAAAAGACTGTACCACCCTCCGAGA

Last Updated on 06.12.2018 16:11