Sequences from confirmation amplicon


Confirmation sequences for line 649H07 and hit At1g23310 are shown.
Line ID 649H07
Locus At1g23310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8271261
Primers used for PCR PF84 - 8474, show primer details
   
Sequence Name 17-K103006-0022-649-H07C-8409
GenBank Accession LN493806 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K103006-0022-649-H07C-8409
GCCTTTACATTGAATATATCCTGACACTTCTTACGGCATACTGACACTTCTTGACGTTTT
CATTCAGAGTATCGTAGTCTAATGCCTTGAGAGCCATTTATGTTCACTCTGAACCAATCC
CTAGCTCACTTCTTCCCTCCTCTACAATTGGAAGAAAACAATTCATCATCAGCAATTGGA
ACCAGAAAACCAGAATTTGGATAAACGAATCAAGATCAAAACTAATTCGACTCTAAGAAC
ACCAAAAGGCCACTAATTTCTGGGTTTCTGTAGCTCAATTTCCAGAACAAATTCGTAATC
ACACAGTGTAAAGGAGACGCCTTTTCAAAAGGGTATTTCAGAATCATTCAAAAGAAGAAG
AATTTGATCAATGATCGATCCAAAGAGAAAGAAAAACCAAATCACAAACCTTCAAA
   
Sequence Name 65-K103007-0022-649-H07C-PF84
GenBank Accession LN511374 [GenBank]
Sequencing primer gene specific primer, PF84: 5'-GTTTGAAGGTTTGTGATTTGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K103007-0022-649-H07C-PF84
ATATTGATAATTCTTCTTCTTTTGATGATTCTGAATACCCTTTTGAAAAGGCGTCTCCTT
TACACTGTGTGATTACGAATTTGTTCTGGAAATTGAGCTACAGAAACCCAGAAATTAGTG
GCCTTTTGGTGTTCTTAGAGTCGAATTAGTTTTGATCTTGATTCGTTTATCCNAATTCTG
GTTTTCTGGTTCCAATTGCTGATGATGAATTGTTTTCTTCCAATTGTAGAGGAGGGAAGA
AGTGAGCTAGGGATTGGTTCAGAGTGAACATAAATGGCTCTCAAGGCATTAGACTACGAT
ACTCTGAATGAAAACGTCAAGAAGTGTCAGTATGCCGTAAGAAGTGTCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTNTTATAAAATGAAAGTACATTTTGATAAAACGACNAATTACGATCCGTCGTAT
TTATNGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTNTATNTCGACGTGTCT
ACGTTCACTCCCAAAT

Last Updated on 06.12.2018 16:11