Sequences from confirmation amplicon


Confirmation sequences for line 650B06 and hit At2g26150 are shown.
Line ID 650B06
Locus At2g26150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11135846
Primers used for PCR HV49 - 8474, show primer details
   
Sequence Name 70-K101271-0022-650-B06C-8409
GenBank Accession LN512345 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K101271-0022-650-B06C-8409
TTCGGACTGAGCCTTTACATTGAATATATCCTGAACAACGAAAATGGAAGAACTGAACGT
GGAAATGGAGGAAGAAACGGTGACGTTTACTGGTTCTGTAGCGGCTTCTTCATCTGTAGG
ATCCTCTTCCTCTCCTAGACCAATGGAAGGGCTTAACGAAACAGGGCCACCACCGTTTCT
GACTAAGACTTACGAAATGGTGGAAGATCCGGCGACGGACACGGTGGTTTCTTGGAGTAA
TGGTCGTAACAGCTTTGTGGTGTGGGATTCTCATAAGTTCTCAACAACTCTCCTTCCACG
TTACTTCAAGCATAGCAATTTCTCAAGTTTTATTCGTCAGCTCAATACTTATGTAAGTAC
CTCTTTGCTTCCTTATATCTACTTAGTCTTGTTTCTGTTTTTGTTTCTAATTTTTAATTT
TGGATTTCTTTAGATGGGTTTCCTATGAGTCTTCAGCTATAAAGGTTAGTTCTTTAAGCT
TTTAAGTGGCTAAATTGAAATTAGATGCATTGAGATTTTGGTCATATGTTAAGAGAATCA
TTTAACATAAACTCTGTTCAATCCTCTGTTTCTGAAGTTCAAAATGTGTTTGGTAGTAAA
AACAGAATCTTGGAATGATAAGTAAGGACTCTGCCTCANGTTCAGTA
   
Sequence Name 70-K101277-0022-650-B06C-HV49
GenBank Accession LN512346 [GenBank]
Sequencing primer gene specific primer, HV49: 5'-CTACTGAACTTGAGGCAGAGTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K101277-0022-650-B06C-HV49
TTTTACACCAACACATTTTGAACTTCAGAAACAGAGGATTGAACAGAGTTCATTTTAAAT
GATTCTCTTAACATATGACCAAAATCTCAATGCATCTAATTTCAATTTAGCCACTTAAAA
GCTTAAAGAACTAACCTTTATAGCTGAAGACTCATAGGAAACCCATCTAAAGAAATCCAA
AATTAAAAATTAGAAACAAAAACAGAAACAAGACTAAGTAGATATAAGGAAGCAAAGAGG
TACTTACATAAGTATTGAGCTGACGAATAAAACTTGAGAAATTGCTATGCTTGAAGTAAC
GTGGAAGGAGAGTTGTTGAGAACTTATGAGAATCCCACACCACAAAGCTGTTACGACCAT
TACTCCAAGAAACCACCGTGTCCGTCGCCGGATCTTCCACCATTTCGTAAGTCTTAGTCA
GAAACGGTGGTGGCCCTGTTTCGTTAAGCCCTTCCATTGGTCTAGGAGAGGAAGAGGATC
CTACAGATGAAGAAGCCGCTACAGAACCAGTAAACGTCACCGTTTCTTCCTCCATTTCCA
CTTTCAGTTCTTCCATTTTCGTTGTTCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11