Sequences from confirmation amplicon


Confirmation sequences for line 652H12 and hit At1g47550 are shown.
Line ID 652H12
Locus At1g47550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17463843
Primers used for PCR SR17 - 8474, show primer details
   
Sequence Name 51-K103544-0022-652-H12C-SR17
GenBank Accession LN507474 [GenBank]
Sequencing primer gene specific primer, SR17: 5'-GTTTGTGTTTGCTTCATGTGTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K103544-0022-652-H12C-SR17
TTTCCAGTTTGCTAAAAAGATTGAGGATTTCATGTATACTATCACCCCTGAAGAGGTTAG
TTGTTAGTTAACTAGTTGTACCATAGTTTCACTTTCTCATTGTGTAAACTATGTGTTGGC
GTGTTACTTTAGGTTTCTAGGGTAAAAAAAAAAATGATACATTTTCTCTATATTTCAAAT
ACCATTCCACCTTGGTTTATCGAAAGTAAAACTCCGGAAAATGTTAAAATCAACCCTGTC
AGGGGTACCCAAATGTTCATTAAAAGTGTATTAATTGTTCACAAACCTTTTTACCAAATC
GTTTTCCAATTCTTCTCTTTCTGTTTAGGTGGACAAATCGATTGCACCAATGTATAAAAA
GTTGCAAAAAAATCTAGCATCAAAGGATTTGCTACCATCGTTGTGGGATAAATGCAAGGT
ACCATTTTAAACTGAAAAGTTGAAAAAAGTAATAAATAACAGTGCATTATTACCTCAGTG
TTTGAATCAATGCGTTGTTGTGTGACCTTAAACAAAAGGAGTTTCTGGACAAGTACAAAA
GCTTCGTGCAGCTCGTACCCAAAGTTTATCCAATCATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACTTGGATCACCAATGAGTATGATGGTCAATATGGAGAAAAAAAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATATC
   
Sequence Name 67-K103545-0022-652-H12C-8409
GenBank Accession LN511794 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K103545-0022-652-H12C-8409
TCGGACTGACCATTTACATTGAAATGATCGGATAAACTTTGGCTACGAGCTGCACGAAGC
TCTCGTACTTGTCCAGAAACTCCTTCTGTTTAAGGTCACACAACAACGCATTGATTCAAA
CACTGAGCTAATAATGCACTGTTATTTATTACTCTCTTCAACTTTTCAGTTTAAACTGGT
ACCTTGCATTTATCCCACAACGATGGTAGCAACTCCTCTGATGCTAGATTCTTCTGCAAC
TTTTTATACATTGCTGCAATCGATTTGTCCACCTAAACAGAAAGAGAAGAATTGGAAAAC
GATCTGGTAAAAAGGTCTGTGAACAATTAATACACTCTTAATGAACATTTGCGTACCCCT
GACAGGCTTGATTTTAACATCTTCCGGAGTTCTACTTTCGATAAACCAAGCTGGAATGGT
ATCTGAAATATAGAGAAAATGTATCATTTTTTTTTTTACCCTAAAAACCTAAAGTAACAC
CCCAACACATATTTTACACAATGAAAAAGTGAAACTATGGTACAACTAGTTAACTAACAA
CTAACCTCTTCAGGGGTGATAGTATACATGAAATCCTCAATCTTTTTAGCAAACTGGAAA
ATTCTTTCAAATTGCTGTAGGGACAAGAGAACACTTGAAGCAAACACAA

Last Updated on 06.12.2018 16:11