Sequences from confirmation amplicon


Confirmation sequences for line 655B10 and hit At3g07880 are shown.
Line ID 655B10
Locus At3g07880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2514027
Primers used for PCR HP80 - 8474, show primer details
   
Sequence Name 75-K033554-022-655-B10C-8409
GenBank Accession LN513176 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K033554-022-655-B10C-8409
GACTTGATCTTCTTGCCGTTACCAAAACCAAAATCCCTAAATTTGCCCTCATTTCCCTCT
GATTAGATCCCTTACCCCGACAAAGACACCAAAATTCTTGAGGGCGAGAGAGCTGATTGA
TGTCTTTGGTATCTGGAGCCAGGGACATGGGGTTCGACGACAACAACAACAACAAGAACA
ACAAGGATGGAGATGATGAGAACTCCTCCTCCAGAACAAGAGCCGACGACGATGCTCTTA
GTCGACAGATGAGCGAATCTTCTCTTTGTGCTACCGAGGAGGAAGAAGACGACGATTCCA
AATTGCAATTGGGTCCTCAGTACACTATCAAAGAACATCTCGAGAAGGACAAGGTTTCTC
CTTTTTGCTTCTTCT
   
Sequence Name 75-K033554-022-655-B10C-HP80
GenBank Accession LN513177 [GenBank]
Sequencing primer gene specific primer, HP80: 5'-AAGAAGAAGCAAAAAGGAGAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K033554-022-655-B10C-HP80
TGAGGACCCATTGCAATTTGGAATCGTCGTCTTCTTCCTCCTCGGTAGCACAAAGAGAAG
ATTCGCTCATCTGTCGACTAAGAGCATCGTCGTCGGCTCTTGTTCTGGAGGAGGAGTTCT
CATCATCTCCATCCTTGTTGTTCTTGTTGTTGTTGTTGTCGTCGAACCCCATGTCCCTGG
CTCCAGATACCAAAGACATCAATCAGCTCTCTCGCCCTCAAGAATTTTGGTGTCTTTGTC
GGGGTAAGGGATCTAATCAGAGGGAAATGAGGGCAAATTTAGGGATTTTGGTTTTGGTAA
CGGCAAGAAGATCAAGTCATGTGGTGATCCGAGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACATTCACGTC
Primers used for PCR AG10 - 3144, show primer details
   
Sequence Name 44-K105356-0022-655-B10C-AG10
GenBank Accession LN505182 [GenBank]
Sequencing primer gene specific primer, AG10: 5'-GGTAGCACCTATTGTTTGATTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K105356-0022-655-B10C-ag10
AAAAAAGGGGCAGAAGCATATGCCCTTGTTTGTCTCATGTCAGGTTGGATCCCAAAATCA
GACCGGATAAAACCGGGATATATCATTAATGCAAGTAATAATGATTGAAGACGAGATTAG
AGGACTAATTCCGTAATTACAAACAAATGATTTTATTGACAATTCAGTGATTATTAAAAA
TAAAAAACATAACAACATTAGTGGTCATTGTCGTTGTCGTGGCTCGAGGCTATCGTCTCT
CCTATGCGCGTGGAAGAAATAGCAATTATGCTTTCTTTTGTATTTGATTATTATCCGTTA
GTGACCCTCACGATCTCTTTATTTGATTTCCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAACAATGAGTATGATAGTTTAAACCGAAGGCGGGAAAC
GACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTGTATAGAG
GAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCA
CATCAATCCA
   
Sequence Name 28-K105357-0022-655-B10C-3144
GenBank Accession LN498843 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K105357-0022-655-B10C-RB31
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGCGATGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCATACTCATTGCTGATCCA
TGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGAAATCAAATAAAG
AGATCGTGAGGGTCACTAACGGATAATAATCAAATACAAAAGAAAGCATAATTGCTATTT
CTTCCACGCGCATAGGAGAGACGATAGCCTCGAGCCACGACAACGACAATGACCACTAAT
GTTGTTATGTTTTTTATTTTTAATAATCACTGAATTGTCAATAAAATCATTTGTTTGTAA
TTACGGAATTAGTCCTCTAATCTCGTCTTCAATCATTATTACTTGCATTAATGATATATC
CCGGTTTTATCCGGTCTGATTTTGGGATCCAACCTAACATGAGACAAACAAGGGCATATG
CTTCTGCCCCTTTTTTTGTGTTTCAATTTAAAAACATATGGAATTTATTGCCACCAATCA
AACAATAGGTGCTAC

Last Updated on 06.12.2018 16:11