Sequences from confirmation amplicon


Confirmation sequences for line 655C10 and hit At3g45150 are shown.
Line ID 655C10
Locus At3g45150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16531288
Primers used for PCR KR16 - 8474, show primer details
   
Sequence Name 04-K030138-022-655-C10C-8409
GenBank Accession LN487792 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K030138-022-655-C10C-8409
AGCCATTTACAATTGAATATGGATCCGAATTGCTCCCCAACTATTTAGATTGACAAAAGA
ACTCGGCTTTAAAACCGATGGTGAAACTGTCAGTTGGCTCCTCCAGAATGCCGAGCCTGC
CATTTTCGCAGCCACGGGACATGGTGTCACCACCACCTCCAATGAAGATATCCAGCCAAA
TAGGAATTTTCCTAGTTACACCTTTAATGGTGATAATATTAGTAATAACGTTTTCCCTTG
TACGGTTGTAAATACTGGTCATCGTCAGATGGTGTTTCCGGTTTCTACAATGACAGATCA
TGCACCTTCAACTAATTACAGTACTATTAGTGATAATTACAATTCCACCTTTAATGGTAA
TGCTACCGCCAGTGATACAACATCAGCAGCAACAACAACAGCCACAACCACAGTTTGAGC
TTGTGAAGGAGGAGACGGCGTCGCTGAGAAGGCCAATTTGGAGTCTTTAAAATAAATATT
GGAGATTTAAGAGTGTTCAGTTCTGGACGTTTTAGTTCGTTTCTGT
   
Sequence Name 04-K030138-022-655-C10C-KR16
GenBank Accession LN487793 [GenBank]
Sequencing primer gene specific primer, KR16: 5'-AAACAGAAACGAACTAAAACGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K030138-022-655-C10C-KR16
TCTTAATCTCCATATTTATTTTAAAGACTCAAATTGGCCTTCTCAGCGACGCCGTCTCCT
CCTTCACAAGCTCAAACTGTGGTTGTGGCTGTTGTTGTTGCTGCTGATGTTGTATCACTG
GCGGTAGCATTACCATTAAAGGTGGAATTGTAATTATCACTAATAGTACTGTAATTAGTT
GAAGGTGCATGATCTGTCATTGTAGAAACCGGAAACACCATCTGACGATGACCAGTATTT
ACAACCGTACAAGGGAAAACGTTATTACTAATATTATCACCATTAAAGGTGTAACTAGGA
AAATTCCTATTTGGCTGGATATCTTCATTGGAGGTGGTGGTGACACCATGTCCCGTGGCT
GCGAAAATGGCAGGCTCGGCATTCTGGAGGAGCCAACTGACAGTTTCACCATCGGTTTTA
AAGCCGAGTTCTTTTGTCAATCTAAATAGTTGGGGAGCAATTCGGATCCATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTAT
AGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
TC

Last Updated on 06.12.2018 16:11