Sequences from confirmation amplicon


Confirmation sequences for line 655D01 and hit At2g40140 are shown.
Line ID 655D01
Locus At2g40140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16773179
Primers used for PCR 28nt - 8474, show primer details
   
Sequence Name 15-K114570-0022-655-D01C-28nt
GenBank Accession LN493076 [GenBank]
Sequencing primer gene specific primer, 28nt: 5'-GCAGAACCAGTAGAAGCATTAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K114570-0022-655-D01C-28nt
CAAGAACAACTCTCCTAGCACAACCAGTCTCATCTTTGCAAGCCGTGTCCTATACTGCGC
CGGGTGAAGCCAAGACTCGAAAACACCGTGCGCGTACTCACACGAATCTCCTTTAGGACA
AGACCCTTTACGAAACTCGGGACAAGGCACACAAGTGTAAGGATACTTCCTAGGATCTCT
CCTCCTTGCATTCTCACCAGGATGAACAAAAGGACATTCAGTCCAATCATGTGAATAAGC
CCTAGAACATGGCTTAACCTTAAAGCTAAACATCCTAAAATCATCAGTTCCATGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTCCAATTCAAAAATGTAGATGTCCGCACGATTATA
   
Sequence Name 02-K114569-0022-655-D01C-8409
GenBank Accession LN487135 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K114569-0022-655-D01C-8409
TGATTTAGGATGTTAGCTTTAGGTTAAGCCATGTTCTAGGGCTTATTCACATGATTGGAC
TGAATGTCCTTTTGTTCATCCTGGTGAGAATGCAAGGAGGAGAGATCCTAGGAAGTATCC
TTACACTTGTGTGCCTTGTCCCGAGTTTCGTAAAGGGTCTTGTCCTAAAGGAGATTCGTG
TGAGTACGCGCACGGTGTTTTCGAGTCTTGGCTTCACCCGGCGCAGTATAGGACACGGCT
TTGCAAAGATGAGACTGGTTGTGCTAGGAGAGTTTGTTTCTTTGCTCATAGACGGGATGA
GTTAAGACCGGTTAATGCTTCTACTGGTTCTGC

Last Updated on 06.12.2018 16:11