Sequences from confirmation amplicon


Confirmation sequences for line 659G04 and hit At1g07640 are shown.
Line ID 659G04
Locus At1g07640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2355946
Primers used for PCR MS78 - 8474, show primer details
   
Sequence Name 15-K026236-022-659-G04C-8409
GenBank Accession LN492811 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K026236-022-659-G04C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTTTTTTTGAATGTTC
TTTCTCTCTTTTTGTAATTTCTCTCTTAAATTCTTCATTGATGTTTTGATTCGTTTTAGT
AATAATTTTGATTCATTTCCAAAAGATATTCCTATTCCCATCTTCTTTTCTAATCCGCTT
TCTTTAATATTCCAACATCTATATATATGGTATTTCTTCCTTTATAAATACGTATTGATA
ATTTGTTACACTAGTTGTGAATTGAGTTTCTTGGTTCTTTCATTAATTTTGATTTGGTTT
TGTAGCCTACGAATTCGAATCATCAGCATCATCTTCAACACCAGCTTAACGAAAATGGAA
GTATAATAA
   
Sequence Name 16-K026236-022-659-G04C-MS78
GenBank Accession LN493280 [GenBank]
Sequencing primer gene specific primer, MS78: 5'-CGTGGCCACTTATTATACTTCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K026236-022-659-G04C-MS78
GTTAAGCTGGTGTTGAAGATGATGCTGATGATTCGAATTCGTAGGCTACAAAACCAAATC
AAAATTAATGAAAGAACCAAGAAACTCAATTCACAACTAGTGTAACAAATTATCAATACG
TATTTATAAAGGAAGAAATACCATATATATAGATGTTGGAATATTAAAGAAAGCGGATTA
GAAAAGAAGATGGGAATAGGAATATCTTTTGGAAATGAATCAAAATTATTACTAAAACGA
ATCAAAACATCAATGAAGAATTTAAGAGAGAAATTACAAAAAGAGAGAAAGAACATTCAA
AAAAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
CTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11