Sequences from confirmation amplicon


Confirmation sequences for line 660A09 and hit At5g63280 are shown.
Line ID 660A09
Locus At5g63280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25368225
Primers used for PCR NL21 - 8474, show primer details
   
Sequence Name 15-K104021-0022-660-A09C-NL21
GenBank Accession LN492946 [GenBank]
Sequencing primer gene specific primer, NL21: 5'-CCATCTTATGACGGTTTTGTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K104021-0022-660-A09C-NL21
AATCTTGTGTTCGCTTCTCTTTTCTCTGTTAAGACAGTAGAAACAGCAATGTGACCAAAG
ATTTCTGAAGATAAGGCAATGATGGAAAATAAAAACAAGTAACACCATACGGTACCTTTG
ATGTAAGAAGACTAGAAGATAAAAGAGTGGGAGTAGCATCAAGGTCAATATTGAAATGGC
GAGGTAGAAGACACCCGATTTCTTCTGCACAAATTAGTATAGACAAAGATGTTAACAGAA
GACTGACATATTCCCTTATATTGGTTTTCACAAGAAACAGATAGCATAGAAGAAGCATAT
GCAAACAGAGTTGCTTTAAAAAATAATTAACAACTTCATTTAGCATGCATAAGTAACTGC
GACGGTTTAAGAGATCATACCTTGCCTCCTCTAGGAAAGGGTTTATCATTTCCAGTGCAG
GTATGAGCATCACAGAATTGGCGCAAAAAGTGTTCTTCGATCATGAAAAATAAAAATCAA
AGACAGTGATTTGAGCACACTTCAAGGAACTATTTAAGAATCATCTCAATGAGCAACTAT
TCACTTGGAAGGTTTCTTAGTAAGGAATCATACCATGACTCANTGGGAAACAACTATGGA
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCG
   
Sequence Name 31-K104022-0022-660-A09C-8409
GenBank Accession LN500136 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K104022-0022-660-A09C-8409
TGAGTCATAGTTGTTTCCCAGTGAGTCATGGTATGATTCCTTACTAAGAAACCTTCCAAG
TGAATAGTTGCTCATTGAGATGATTCTTAAATAGTTCCTTGAAGTGTGCTCAAATCACTG
TCTTTGATTTTTATTTTTCATGATCGAAGAACACTTTTTGCGCCAATTCTGTGATGCTCA
TACCTGCACTGGAAATGATAAACCCTTTCCTAGAGGAGGCAAGGTATGATCTCTTAAACC
GTCGCAGTTACTTATGCATGCTAAATGAAGTTGTTAATTATTTTTTAAAGCAACTCTGTT
TGCATATGCTTCTTCTATGCTATCTGTTTCTTGTGAAAACCAATATAAGGGAATATGTCA
GTCTTCTGTTAACATCTTTGTCTATACTAATTTGTGCAGAAGAAATCGGGTGTCTTCTAC
CTCGCCATTTCAATATTGACCTTGATGCTACTCCCACTCTTTTATCTTCTAGTCTTCTTA
CATCAAAGGTACCGTATGGTGTTACTTGTTTTTATTTTCCATCATTGCCTTATCTTCAGA
AATCTTTGGTCACATTGCTGTTTCTACTGTCTTTAACAGAGAANAGAGAAGCGGAACACA
AGATTTGCGTCGTATTATAAAATCTGGAAAAAAAACAAAACCGT
Primers used for PCR CB35 - CR3S, show primer details
   
Sequence Name 40-K117832-0022-660-A09C-CB35
GenBank Accession LN503857 [GenBank]
Sequencing primer gene specific primer, CB35: 5'-TGCAAGAAAAGTTTCAATGATGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K117832-0022-660-A09C-CB35
TCTTTTAATACGGTATGTCTTATATGTTTCTCATCTTGAGGATAATGAAATTCTGTTATT
TAGTGATATTTTGCTTGTTTTCAGACTGATACAAAGTGTTTAGCCGACCTGTGTGGTGCA
TTGCATTGTGACTTTGTATTGAGCTCTAAGAAACCCAAGAGCAAGTGCAACCCTCCTGCT
GTTGCCAAAAACCGTCATCTCTGCGAGGTTTCTTTCTTTGATTCTCTTTTCATTATTATT
TGCTTTTTCCAGCTCTGGTTCCATGACTTGTTATTATTATCTTTCGATACTAATTTTACT
TGTTTATTCACTTAGAGCGTTGCCAATAGTTGTTTCCCAGTGAGTCAAGGTCCCTCAGCT
AAGAAAATTGGTAATTACTCTTTCTTTTTCTCCATATTGACCATCATACATGAAGCCATT
CACCTGGATTTTGGTTTTAGGAATTAGAAATTTTATTGATAGAAGTATTTTACAAATACA
AATACATACTAAGGGTTTCTTATATGCTCAA
   
Sequence Name 12-K117831w-0022-660-A09C-CR3S
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K117831w-0022-660-A09C-CR3S
CATAAATTTCTATTCCTAAACCAAAATCCAGGTGAATGGCTTCATGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTCTTAGCTGAGGGACCTTGACTCACTGGGA
AACAACTATTGGCAACGCTCTAAGTGAATAAACAAGTAAAATTAGTATCGAAAGATAATA
ATAACAAGTCATGGAACCAGAGCTGGAAAAAGCAAATAATAATGAAAAGAGAATCAAAGA
AAGAAACCTCGCAGAGATGACGGTTTTTGGCAACAGCAGGAGGGTTGCACTTGCTCTTGG
GTTTCTTAGAGCTCAATACAAAGTCACAATGCAATGCACCACACAGGTCGGCTAAACACT
TTGTATCAGTCTGAAAACAAGCAAAATATCACTAAATAACAGAATTTCATTATCCTCAAG
ATGAGAAACATATAAGACATACCGTATTTAAAAGATTATAATGTCTAGTACTGAAATGCT
TGTCAAGAAATTTCTCATCATTGAAACTTTTCTTGCA

Last Updated on 06.12.2018 16:11