Sequences from confirmation amplicon


Confirmation sequences for line 670F02 and hit At5g17040 are shown.
Line ID 670F02
Locus At5g17040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5605568
Primers used for PCR HV18 - 8474, show primer details
   
Sequence Name 21-K031868-022-670-F02C-8409
GenBank Accession LN495554 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K031868-022-670-F02C-8409
TTTACAATTGAATATATCCTGATGAGAAACAATCGCTCACTGCTCATCTCTACACAAATC
GATTGTGTGGGAAATTGGAATGACGATTAGTAGTGGAGTCTTCACGAAGGATGGATTTGA
GGAGAGTTTGGATCGGGTTTTGGTTCAAGATGATGGCAAGAAGATGAAGTTTAATGCCAA
GAAGCTTAAAGAACTAGCACAAGAAGCTGTCTCTACGGAAGGAAGCTCCTTTGAGAATTT
CAAAGGTTTGTTGGACGAAGTTATGAAAGTTTGAAGCAAATAGACTTTGGATAAATAAAC
AAATAAACATGTTCTGTTTTGGAATGAAAATGAAAAGACCTTTTTTGTAAAAAAAAAGTG
AAAGTGAGAATCTTTATCCTTTGTTTTTATTTTTTATTTTTTTCTGTTATGGTAACTTTG
TAATTAATATATACATATCTAAACAAATTGTATGTCGGTAGATATATGTTTTGAGAAAAA
AATATCTATCGTGTAGACTAGTCTCCATATAAAAAAATATCTATACGTCTGTCGATCTAG
GGGCTTACTTTAGAACATTGAATGAAAGACAGATGAGACTTGATGAGAAAACTCACCATA
TAATATAAGAACCACAAATTCTACCTACCAAAA
   
Sequence Name 22-K031868-022-670-F02C-HV18
GenBank Accession LN496000 [GenBank]
Sequencing primer gene specific primer, HV18: 5'-TCCCTGCTATATTGTTTTGGTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K031868-022-670-F02C-HV18
CATCAGTCTCATCTGTCTTTCATTCAATGTTCTAAAGTAAGCCCCTAGATCGACAGACGT
ATAGATATTTTTTTATATGGAGACTAGTCTACACGATAGATATTTTTTTCTCAAAACATA
TATCTACCGACATACAATTTGTTTAGATATGTATATATTAATTACAAAGTTACCATAACA
GAAAAAAATAAAAAATAAAAACAAAGGATAAAGATTCTCACTTTCACTTTTTTTTTACAA
AAAAGGTCTTTTCATTTTCATTCCAAAACAGAACATGTTTATTTGTTTATTTATCCAAAG
TCTATTTGCTTCAAACTTTCATAACTTCGTCCAACAAACCTTTGAAATTCTCAAAGGAGC
TTCCTTCCGTAGAGACAGCTTCTTGTGCTAGTTCTTTAAGCTTCTTGGCATTAAACTTCA
TCTTCTTGCCATCATCTTGAACCAAAACCCGATCCAAACTCTCCTCAAATCCATCCTTCG
TGAAGACTCCACTACTAATCGTCATTCCAATTTCCCACACAATCGATTTGTGTAGAGATG
AGCAGTGAGCGATTGTTTCTCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11