Sequences from confirmation amplicon


Confirmation sequences for line 671E09 and hit At3g12100 are shown.
Line ID 671E09
Locus At3g12100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3857296
Primers used for PCR GG27 - 8474, show primer details
   
Sequence Name 31-K035981-022-671-E09C-8409
GenBank Accession LN500068 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K035981-022-671-E09C-8409
TGAAGCCATTTTTTTACTTCGGCAATACTAAAATCAACAGTCAGTCGTACAAAGAAACTC
TGCTTATACAGTAAAGTCAATGGGCCACTGTTCTAAGCCCATATATAATTTTAGAAGCCC
ATAGAATACAAAAGAGTCAAGAAGCATTGACCGCACAAGAAAAAAACAATTGTTAAAAAG
GGTTGGTTAGTGTGTATGTATATATGAAATGCAACAAACATTATACAGCCCATTAAATAT
GGTTGTTATAGGTAGATGTCCCCATTAAGGAACTTTATCCAGCCCATTAAATTACTTTAC
AGAGTAAAAGAGAGAGAGAAGATTTACAGTTACGTTACCAAATTTTCGAAATGATTTAAT
TAGTAATAAATAAATAATTAAATGTCAGTTACTCTCTTTAGAAAGCTAAATAAGACAGCT
GTTTCCACCAACAACGTGACTGGTCGTGGGGTCCTCCTTCGTTCAAAGTGATATTCAGAA
ATCAACGGCTGAGATCTTCTCCATCAATATTTATTACGGGCCTATTCCTTCCTTTTTTAA
ACTTCAATTCTCCGGCTCACATTCTCTTCTTCATTCGCTCCGTTTCTC
   
Sequence Name 32-K035981-022-671-E09C-GG27
GenBank Accession LN500491 [GenBank]
Sequencing primer gene specific primer, GG27: 5'-TAGTTTTTGAGAGAGAAACGGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K035981-022-671-E09C-GG27
GCCGGAGATTGAAGTTTAAAAAGGAAGGAATAGGCCCGTAATAAATATTGATGGAGAAGA
TCTCAGCCGTTGATTTCTGAATATCACTTTGAACGAAGGAGGACCCCACGACCAGTCACG
TTGTTGGTGGAAACAGCTGTCTTATTTAGCTTTCTAAAGAGAGTAACTGACATTTAATTA
TTTATTTATTACTAATTAAATCATTTCGAAAATTTGGTAACGTAACTGTAAATCTTCTCT
CTCTCTTTTACTCTGTAAAGTAATTTAATGGGCTGGATAAAGTTCCTTAATGGGGACATC
TACCTATAACAACCATATTTAATGGGCTGTATAATGTTTGTTGCATTTCATATATACATA
CACACTAACCAACCCTTTTTAACAATTGTTTTTTTCTTGTGCGGTCAATGCTTCTTGACT
CTTTTGTATTCTATGGGCTTCTAAAATTATATATGGGCTTAGAACAGTGGCCCATTGACT
TTACTGTATAAGCAGAGTTTCTTTGTACGACTGACTGTTGATTTTAGTATTTGCCGAAGT
AAAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
ATTCACGCCCCAAATGGGGA
Primers used for PCR NC34 - 8474, show primer details
   
Sequence Name 41-K035982-022-671-E09C-8409
GenBank Accession LN503993 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K035982-022-671-E09C-8409
GCCATTTTTTACTTCGGCAAATACTAAAATCAACAGTCAGTCGTACAAAGAAACTCTGCT
TATACAGTAAAGTCAATGGGCCACTGTTCTAAGCCCATATATAATTTTAGAAGCCCATAG
AATACAAAAGAGTCAAGAAGCATTGACCGCACAAGAAAAAAACAATTGTTAAAAAGGGTT
GGTTAGTGTGTATGTATATATGAAATGCAACAAACATTATACAGCCCATTAAATATGGTT
GTTATAGGTAGATGTCCCCATTAAGGAACTTTATCCAGCCCATTAAATTACTTTACAGAG
TAAAAGAGAGAGAGAAGATTTACAGTTACGTTACCAAATTTTCGAAATGATTTAATTAGT
AATAAATAAATAATTAAATGTCAGTTACTCTCTTTAGAAAGCTAAATAAGACAGCTGTTT
TCC
   
Sequence Name 42-K035982-022-671-E09C-NC34
GenBank Accession LN504379 [GenBank]
Sequencing primer gene specific primer, NC34: 5'-TGGTGGAAACAGCTGTCTTATTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K035982-022-671-E09C-NC34
TTTAATTATTTATTTATTACTAATTAAATCATTTCGAAAATTTGGTAACGTAACTGTAAA
TCTTCTCTCTCTCTTTTACTCTGTAAAGTAATTTAATGGGCTGGATAAAGTTCCTTAATG
GGGACATCTACCTATAACAACCATATTTAATGGGCTGTATAATGTTTGTTGCATTTCATA
TATACATACACACTAACCAACCCTTTTTAACAATTGTTTTTTTCTTGTGCGGTCAATGCT
TCTTGACTCTTTTGTATTCTATGGGCTTCTAAAATTATATATGGGCTTAGAACAGTGGCC
CATTGACTTTACTGTATAAGCAGAGTTTCTTTGTACGACTGACTGTTGATTTTAGTATTT
GCCGAAGTAAAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACATTCACTCCCAAAAT

Last Updated on 06.12.2018 16:11