Sequences from confirmation amplicon


Confirmation sequences for line 675H03 and hit At1g32580 are shown.
Line ID 675H03
Locus At1g32580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11784098
Primers used for PCR 05en - 8474, show primer details
   
Sequence Name 13-K110753-0022-675-H03C-05en
GenBank Accession LN492092 [GenBank]
Sequencing primer gene specific primer, 05en: 5'-CTCTTCATCAATCTCACAACCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K110753-0022-675-H03C-05en
GATACATTATAAATCTTCTTCTAGCTTCTTCCTCACTGATTAAACAAACAACAAGAGATT
ATTAATTACAATAACTAAATGCTTCTTCTAACTAATGAATCAACGAAATTGAAAGAAACC
CTAACCTGCCGATAATTTTAGCTAGGGTTTGAACATAACAATCAATCATTTGCTGCTTAG
TAGCGTTTTCGCCTCCAGGTTTGTCCATTACAATCAACCAATGCTCATAATCGCAGCCAG
GAAACAACGGCGCCATCTCAGTGGGTGCTCGGTCGCTGAAATTCGAACCGGATTTTAGAG
GAGAGTACGATCCACCGGACCTATCCATTCGAGTTCGAATCGTTGTGAAACGATTCAAGG
AAGAGATGAATCCGACGGCATGGGAGAACACCGGGGTTGATCGACGGGAGAGGATATAAG
AAGGGGAAGGAGTGGTGGCTCCGGAGGTGGAGATTAAGCGCTTGGTGATGCGTGACGCTG
TGGAGCGAGCTAGGGTTTTCGCCATTGGAGATCAGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTACG
   
Sequence Name 05-K110750-0022-675-H03C-8409
GenBank Accession LN488409 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K110750-0022-675-H03C-8409
TCGGACTGAGCATTTCATTGAATATATCCTGATCTCCATGGCGAAAACCCTAGCTCGCTC
CACAGCGTCACGCATCACCAAGCGCTTAATCTCCACCTCCGGAGCCACCACTCCTTCCCC
TTCTTATATCCTCTCCCGTCGATCAACCCCGGTGTTCTCCCATGCCGTCGGATTCATCTC
TTCCTTGAATCGTTTCACAACGATTCGAACTCGAATGGATAGGTCCGGTGGATCGTACTC
TCCTCTAAAATCCGGTTCGAATTTCAGCGACCGAGCACCCACTGAGATGGCGCCGTTGTT
TCCTGGCTGCGATTATGAGCATTGGTTGATTGTAATGGACAAACCTGGAGGCGAAAACGC
TACTAAGCAGCAAATGATTGATTGTTATGTTCAAACCCTAGCTAAAATTATCGGCAGGTT
AGGGTTTCTTTCAATTTCGTTGATTCATTAGTTAGAAGAAGCATTTAGTTATTGTAATTA
ATAATCTCTTGTTGTTTGTTTAATCAGTGAGGAAGAAGCTAAGAAGAAGATTTATAATGT
ATCGTGTGAAAGGTATTTTGGATTTGGTTGTGAGATTGATGAAGAG

Last Updated on 06.12.2018 16:11