Sequences from confirmation amplicon


Confirmation sequences for line 676A09 and hit At3g02830 are shown.
Line ID 676A09
Locus At3g02830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:615454
Primers used for PCR JV13 - 8474, show primer details
   
Sequence Name 69-K117894-0022-676-A09C-JV13
GenBank Accession LN512278 [GenBank]
Sequencing primer gene specific primer, JV13: 5'-CTACCTCAGGGCAACAGTCTTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K117894-0022-676-A09C-JV13
CAGATCCGTCTAGCTACGCTTCCTTAATCATGCCTCAAGGAGTTGTCCCGGTTCAAGGAT
GGAACCCTTACAGTGTAAGTTTTCTAAACAACATTGCTTATACTCTTCAGACTTTATATG
ACTTGGTTTTGATTTCTGTCGAGTGTTTCTCGGGGTTTCCATTTCGTTAGGGTCAGCTCG
GCTCAGTTTCTCCTTCAGGTACCGGAAATGATCAAAACTACAGAAATTTACAGCAGAACG
AGACAATAGAGTCAGGCTCTCAGTCTCAAGGATCATTCTCGGGTTACAATCCGGGATCCT
CTGTTCCGCTAGGAGGGTATTATGCATTGCCGAGGGAAAATGTGTTCCCGGAGACATTCC
AATTCAATTGAAGCCATTTGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATT
   
Sequence Name 58-K117893-0022-676-A09C-8409
GenBank Accession LN509821 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K117893-0022-676-A09C-8409
CATTTCATTGAATATATCCTGCCAATGGCTTCAATTGAATTGGAATGTCTCCGGGAACAC
ATTTTCCCTCGGCAATGCATAATACCCTCCTAGCGGAACAGAGGATCCCGGATTGTAACC
CGAGAATGATCCTTGAGACTGAGAGCCTGACTCTATTGTCTCGTTCTGCTGTAAATTTCT
GTAGTTTTGATCATTTCCGGTACCTGAAGGAGAAACTGAGCCGAGCTGACCCTAACGAAA
TGGAAACCCCGAGAAACACTCGACAGAAATCAAAACCAAGTCATATAAAGTCTGAAGAGT
ATAAGCAATGTTGTTTAGAAAACTTACACTGTAAGGGTTCCATCCTTGAACCGGGACAAC
TCCTTGAGGCATGATTAAGGAAGCGTAGCTAGACGGATCTTGCCAACGAGGGCTGGCAAT
AAAAGAAGCTCTTGACCAAGGATAAGACTGTTGCCCTGAGGTAGAAAANAACCAC
Primers used for PCR 25dk - 8474, show primer details
   
Sequence Name 52-K118366-0022-676-A09C-25dk
GenBank Accession LN507956 [GenBank]
Sequencing primer gene specific primer, 25dk: 5'-GAAGCTGTGTTGTCGTATGTGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K118366-0022-676-A09C-25dk
AATTTGCAGATTCCATAGCGTGTATAGAACACACANCAACGGTTCTCCCTATCATTTTGA
GAAAGAAAAAAAATCAAACAATGCAGAGATTTAATCGAAGAGAACCAAGTAATTATGCAC
AAGTCTTTGAGCTTACCGGGCGTAAGGGGAGGCCAATGGAGCTTAAGAGACAATCAGGAG
GAGGAGCTTGTCTGTCTCTAGGATGGTGAAATTTGCAAACTGTGCCAAATTTGCAGTCAC
CTGTCTTCATGTAGAATTGGCATTCGGCAATTGTAACCCGGAACAAATGAACCTGTCTTT
GAATATTGAAGCCATTTGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 73-K118365-0022-676-A09C-8409
GenBank Accession LN512971 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K118365-0022-676-A09C-8409
CATTTACATTGAATATATCCTGCCAAATGGCTTCAATATTCAAAGACAGGTTCATTTGTT
CCGGGTTACAATTGCCGAATGCCAATTCTACATGAAGACAGGTGACTGCAAATTTGGCAC
AGTTTGCAAATTTCACCATCCTAGAGACAGACAAGCTCCTCCTCCTGATTGTCTCTTAAG
CTCCATTGGCCTCCCCTTACGCCCGGTAAGCTCAAAGACTTGTGCATAATTACTTGGTTC
TCTTCGATTAAATCTCTGCATTGTTTGATTTTTTTTCTTTCTCAAAATGATAGGGAGAAC
CGTTGTGTGTGTTCTATACACGCTATGGAATCTGCAAATTTGGTCCAAGCTGTAAATTTG
ATCACCCAATGCGAGTATTCACATACGACACNACAGCT

Last Updated on 06.12.2018 16:11