Sequences from confirmation amplicon


Confirmation sequences for line 679F09 and hit At4g30030 are shown.
Line ID 679F09
Locus At4g30030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14683186
Primers used for PCR NG86 - 8474, show primer details
   
Sequence Name 18-K102364-0022-679-F09C-8409
GenBank Accession LN494248 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K102364-0022-679-F09C-8409
TTACATTGAAATATTCACTACCTGTATCTATGAGTAAGAGTTGCGGGACAGGTGGATTGC
CGATAGAAATATTTGCAAGGAATGCTGCTGGATTCGGAATAGGAGTGACATGTGAGACGG
TCCACAAGTTATCTAGGCGAGAAGCGGGGGTTGACTTGGAATGCAAGTAACCGATTTTGA
TTTTCGATGATTCTTGGGTTTTGGTTCTTAGCGTAAGTCGGGAATGCGAGGTGCATTCCT
TTGAACAAGTGAGAGCGAATATAATGATAAGAAGGGAGGCACGAATACGTAATGGTAGTA
GAGTGATGGCCATTTGATAATCAAGAATGTGGTTTTCTTCCAATCCTTTAAATATGAGTC
TAAAGAAGAATGGCATTGATCACTCTACTACCATTACGTATTCGTGCCTCCCTTCTTATC
ATTATATTCGCTCTCACTTGT
   
Sequence Name 18-K102365-0022-679-F09C-NG86
GenBank Accession LN494249 [GenBank]
Sequencing primer gene specific primer, NG86: 5'-AGAGTGATCAATGCCATTCTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K102365-0022-679-F09C-NG86
GAGAACCCATTCTTGATTATCAAATGGCCATCACTCTACTACCATTACGTATTCGTGCCT
CCCTTCTTATCATTATATTCGCTCTCACTTGTTCAAAGGAATGCACCTCGCATTCCCGAC
TTACGCTAAGAACCAAAACCCAAGAATCATCGAAAATCAAAATCGGTTACTTGCATTCCA
AGTCAACCCCCGCTTCTCGCCTAGATAACTTGTGGACCGTCTCACATGTCACTCCTATTC
CGAATCCAGCAGCATTCCTTGCAAATATTTCTATCGGCAATCCACCTGTCCCGCAACTCT
TACTCATAGATACAGGTAGTGAATATTTTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11