Sequences from confirmation amplicon


Confirmation sequences for line 683G07 and hit At5g43850 are shown.
Line ID 683G07
Locus At5g43850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17628041
Primers used for PCR NL29 - 8474, show primer details
   
Sequence Name 33-K104023-0022-683-G07C-NL29
GenBank Accession LN500970 [GenBank]
Sequencing primer gene specific primer, NL29: 5'-TTTGTACCTTGATGTAGTTGCTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K104023-0022-683-G07C-NL29
GCAGGAGGACATGAGATCGCCAGGTTGCATCCAGATACGGATCCAACGATCATCCTTGTC
CCTAACATCAAAGTAGCCACTTCCTGCTAGGCAGTACCGAATCTCTTCGTCCTTGTGAAT
GTGTTCTGTGAAAAAGTTCTTCAGCTTTTCTTCGTAGTTGCTGACTTTCTCGGGACACAG
ATCCAGCAAATCCTAAAATGAAGATTAACCAAAGTAATCATTCTGCTTGAGAAAATTAGT
TTATAGCTCATAAGTTACAATGCTACACACCACACAAATAGCTTAATTATTTTTAACGTA
AACCAAACGTTTTAATGTTCCCTTAATACTCAATTACAGAGAAGCCTTCTTCCTTAATAA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAC
   
Sequence Name 49-K104024-0022-683-G07C-8409
GenBank Accession LN506821 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K104024-0022-683-G07C-8409
GCCATTTTATAAGGAAGAAGGCTTCTCTGTAATTGAGTATTAAGGGAACATTAAAACGTT
TGGTTTACGTTAAAAATAATTAAGCTATTTGTGTGGTGTGTAGCATTGTAACTTATGAGC
TATAAACTAATTTTCTCAAGCAGAATGATTACTTTGGTTAATCTTCATTTTAGGATTTGC
TGGATCTGTGTCCCGAGAAAGTCAGCAACTACGAAGAAAAGCTGAAGAACTTTTTCACAG
AACACATTCACAAGGACGAAGAGATTCGGTACTGCCTAGCAGGAAGTGGCTACTTTGATG
TTAGGGACAAGGATGATCGTTGGATCCGTATCTGGATGCAACCTGGCGATCTCATTGTCC
TTCCTGCCGGAATCTACCACCGGTTCACACTCGACGCCNGCAACTACATCAGGGTACA
Primers used for PCR NL30 - 8474, show primer details
   
Sequence Name 36-K118809-0022-683-G07C-NL30
GenBank Accession LN502416 [GenBank]
Sequencing primer gene specific primer, NL30: 5'-TTGAACCCTGAGAACTACGAGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K118809-0022-683-G07C-NL30
GGTTACGATTACATGGTACGCATATTATCTCTTCCTGATCACTGTTTTTTCTTAACTTAG
GAACACTCATGCTCATCATAAATGCCAATTGAATGTCTCTGTTTTTTAACTTGTTGCATA
TCGTTGCATGTTGATCTCATGAGATTAACGATTGTGACATATACTAGACACAGTAGGAGT
GTAGGACTACATAATGAAGGTAGTACTCAAACCTTACAAAACCAGCCTATGAAGAAAGAA
GCCTTATGAGTATTAGAGTACAGGATTTGCTACGGCTTATGTCTTTCTGATCACCCTTTT
TTAACTCATGCTCACCATAAGCGGCTATTGATGTTTCAGTTCCTTTACTTGTTATCTCTG
CACGCTAATCTCATGAAAAAAAAAAACATGTATCAAAGGACAGTAGGACTATAATCTTGC
ATAACCTTTAAGGGGTAGTATGACTACAAACTTTTTAAAAATAGATCACCAATGAGTAAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCATTTCAAAAATGTAAAATTCC
   
Sequence Name 04-K118808-0022-683-G07C-8409
GenBank Accession LN488125 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K118808-0022-683-G07C-8409
ATTTACTCATTGCTGATCTATTTCTAAAATGTTTGTAGTCATACTACCCCTTAATGGTTA
TGCAAGATTATAGTCCTACTGTCCTATGATACATGTTTTTTTTTTTCATGAAATTAGCGT
GCAAAGATAACAAGTAAAGGAACTGAAACATCAATACCCGCTTATGGTGAGCATGAGTTA
AAAAAGGGTGATCAAAAAGACATAAGCCGTACCAAATCCGGTACTCTAATACTCAAAAGG
CTTCTTTCTTCAAAGGCGGGTTTTGTAAGGTTTGAGTACTACCTTCATTATGTAGCCCTA
CACTCCTACGGGGTCTAGTATATGTCACAATCGTTAATCTCAGGAAATCAACATGCAACG
ATTTGCAACAAGTTAAAAAACAAAGACTTTCAATTGGCATTTATAATGAGCATGAGTGTT
CCTAATTTAAAAAAAAACAGTGATCAGGAAAAAATAATATGCGTACCAGGTAATCGTAAC
CCCTGTCTTCTCTAATCTGGCTAAGTTCAAAATCATTCTCGTAGTTCTCAGGGGTTCAAA

Last Updated on 06.12.2018 16:11