Sequences from confirmation amplicon


Confirmation sequences for line 684H02 and hit At5g45110 are shown.
Line ID 684H02
Locus At5g45110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18230064
Primers used for PCR CP35 - 8474, show primer details
   
Sequence Name 57-K031139-022-684-H02C-CP35
GenBank Accession LN509316 [GenBank]
Sequencing primer gene specific primer, CP35: 5'-TTTCGAAAACTGAGAAACCAAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K031139-022-684-H02C-CP35
GAGCTGTTGCTCTGAAGCTTTCTTGTATTTCTTGAGTTATATATATACTGGGAGATTAAA
GCCTTTTCCATTGGAGGTTTCGACTTGTGTTGATCCAGTTTGTTCTCATGATTGTTGTCG
ACCTGCCATTGATTTTGTTGTTCAATTGATGTATGCTTCCTCTGTTCTCCAAGTGCCTGA
GCTAGTTTCATCTTTTCAGGTGTGTGAAAAGTCTTCTTTTTTCTTCGGTCTCTTTGTTTC
TTAATTCATAGACCTCAATGAGAACTAAAAGGTTTGTGTGCTTGCAGCGGCGGCTTTGTA
ACTTTGTGGAGAAGACCCTTGTTGAGAATGTTCTTCCCATTCTTATGGTTGCTTTCAATT
GTAAGTTGACTCAGCTTCTTGATCAGTGTATTGAGAGAGTGGCGAGGTCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTA
TTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTC
TACATTC
   
Sequence Name 22-K031139-022-684-H02C-8409
GenBank Accession LN495993 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K031139-022-684-H02C-8409
ATTTACATTGAATATATCCTGACCTCGCCACTCTCTCAATACACTGATCAAGAAGCTGAG
TCAACTTACAATTGAAAGCAACCATAAGAATGGGAAGAACATTCTCAACAAGGGTCTTCT
CCACAAAGTTACAAAGCCGCCGCTGCAAGCACACAAACCTTTTAGTTCTCATTGAGGTCT
ATGAATTAAGAAACAAAGAGACCGAAGAAAAAAGAAGACTTTTCACACACCTGAAAAGAT
GAAACTAGCTCAGGCACTTGGAGAACAGAGGAAGCATACATCAATTGAACAACAAAATCA
ATGGCAGGTCGACAACAATCATGAGAACAAACTGGATCAACACAAGTCGAAACCTCCAAT
GGAAAAGGCTTTAATCTCCCAGTATATATATAACTCAAGAAATACAAGAAAGCTTCATGA
GCAACAGCTCCATAAGGTAACATCTCTCTCAACTGATACTTTGGTTTCT

Last Updated on 06.12.2018 16:11