Sequences from confirmation amplicon


Confirmation sequences for line 688F07 and hit F15O5 are shown.
Line ID 688F07
Locus F15O5 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25997126
Primers used for PCR KF40 - 8474, show primer details
   
Sequence Name 41-K103355-0022-688-F07C-KF40
GenBank Accession LN504045 [GenBank]
Sequencing primer gene specific primer, KF40: 5'-ACGCGATTGACATCAATAATCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K103355-0022-688-F07C-KF40
TATTTTTTATTTTTATTTCTCTCTAGAGTTTTACACTGTCAAATTACCTATCTAGTTCTT
TTTTTTCTTTTTCTTTTACGACTACTAGCTAGAGAGACATTTGCACATACAGTAAAGGAA
TTTATTTTATGTAGGGGTTCAAACACTTGGGAAGAAGAGGATTTACAGACTTTTTTAACA
TGAAATTATTGGATGACTCTTCTTCCCCTAAGCTAAATTTACAGACTTTTTGTTGTTTGG
AAAAAGTTCTTCATATAAAAAACAATATGAACAGTATTACAGTAACCTGAGCTGGAATAA
CTTGAGCAGTAGTTTGTTTGGGCTTTTTCTTTGATCAAAAACTTTCTATTAAAATGCATC
AGAAGAAAAATCATTACATGGCTCAGCCCAATGTTTTGGTTTCTCATACAGCCCAATACA
TGCTCATTACTAGTATTGCTACAGCCCAATATATTCTCATTAGTCATTACTAGTATCGGT
GGAATGAACTAAATCCAATCAACGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTA
   
Sequence Name 57-K103356-0022-688-F07C-8409
GenBank Accession LN509387 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K103356-0022-688-F07C-8409
GCCATTTACGTTGATTGGATTTAGTTCATTCCACCGATACTAGTAATGACTAATGAGAAT
ATATTGGGCTGTAGCAATACTAGTAATGAGCATGTATTGGGCTGTATGAGAAACCAAAAC
ATTGGGCTGAGCCATGTAATGATTTTTCTTCTGATGCATTTTAATAGAAAGTTTTTGATC
AAAGAAAAAGCCCAAACAAACTACTGCTCAAGTTATTCCAGCTCAGGTTACTGTAATACT
GTTCATATTGTTTTTTATATGAAGAACTTTTTCCAAACAACAAAAAGTCTGTAAATTTAG
CTTAGGGGAAGAAGAGTCATCCAATAATTTCATGTTAAAAAAGTCTGTAAATCCTCTTCT
TCCCAAGTGTTTGAACCCCTACATAAAATAAATTCCTTTACTGTATGTGCAAATGTCTCT
CTAGCTAGTAGTCGTAAAAGAAAAAGAAAAAAAAGAACTAGATAGGTAATTTGACAGTGT
AAAACTCTNGAGAGAAATAAAAAATAAAAAAATTAGGAAGATCAACGGAAATTGCGTTGA
CTATGATTATTGATTNAAATCGCG

Last Updated on 06.12.2018 16:11