Sequences from confirmation amplicon


Confirmation sequences for line 692A05 and hit At2g31320 are shown.
Line ID 692A05
Locus At2g31320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13355373
Primers used for PCR BE77 - 8474, show primer details
   
Sequence Name 15-K029122-022-692-A05C-8409
GenBank Accession LN492838 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K029122-022-692-A05C-8409
TGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATCGCAATGCAGCTTCTTATAC
TTATCATCAAGAGATTCGGTGCTATCAACATCAAAGCCAACTATTCTTGAAGCTATTTCG
ATATCCTGCAGAGCCTCGAGCATTTTCACCTGTGACACACAGATACAATACCTCTTTGTA
ACACAGAAAAAGAGGCTAGGAATTAAAAAACAACACCATGGTTCATGTTCTATGGCAAGT
ACCTTTGACTTAAAGTCATCTTCATCTCGGATAATATGAGGATGAATAGAAGGGATCATG
GTAAAAAATCTGTTACTAGCATCAACAAGCAAGCTTTCTTTCATAGTAGGCTGGGGGTCG
CTTTCAGTCAATAGCCTCTGTATCTCCGTCAATGCCTCAAAACCTTTAGAAGAACAAACA
AGAAAAAACATAATAGAATGTAATGTAGAATTAACATTTTATTATTTTTATCATATAAAA
AAGTAAACTAGAGAAGTAGGAGTTACCCTTCTGTATATTATGTTTGCTGAGCTTCCCAAG
TGGCATCTCTGACATATTTATCTCGAACTCCATCATTGCAGATCTGCGGAAGTAATTATT
AGGTTACTTCATACAGTTTCTAGGGAGAGAA
   
Sequence Name 16-K029122-022-692-A05C-BE77
GenBank Accession LN493307 [GenBank]
Sequencing primer gene specific primer, BE77: 5'-GTCTTCTTCTCCTCTTCTCTCCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K029122-022-692-A05C-BE77
TGTATGAAGTAACCTAATAATTACTTCCGCAGATCTGCAATGATGGAGTTCGAGATAAAT
ATGTCAGAGATGCCACTTGGGAAGCTCAGCAAACATAATATACAGAAGGGTAACTCCTAC
TTCTCTAGTTTACTTTTTTATATGATAAAAATAATAAAATGTTAATTCTACATTACATTC
TATTATGTTTTTTCTTGTTTGTTCTTCTAAAGGTTTTGAGGCATTGACGGAGATACAGAG
GCTATTGACTGAAAGCGACCCCCAGCCTACTATGAAAGAAAGCTTGCTTGTTGATGCTAG
TAACAGATTTTTTACCATGATCCCTTCTATTCATCCTCATATTATCCGAGATGAAGATGA
CTTTAAGTCAAAGGTACTTGCCATAGAACATGAACCATGGTGTTGTTTTTTAATTCCTAG
CCTCTTTTTCTGTGTTACAAAGAGGTATTGTATCTGTGTGTCACAGGTGAAAATGCTCGA
GGCTCTGCAGGATATCGAAATAGCTTCAAGAATAGTTGGCTTTGATGTTGATAGCACCGA
ATCTCTTGATGATAAGTATAAGAAGCTGCATTGCGATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
Primers used for PCR FN52 - CR3S, show primer details
   
Sequence Name 27-K117832-0022-692-A05C-FN52
GenBank Accession LN498580 [GenBank]
Sequencing primer gene specific primer, FN52: 5'-TAGGAGCATCTTATTGCCAAGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K117832-0022-692-A05C-FN52
ACTCTCCTTCCTTTCAGAGCAAAACTTCCTCTAGCTCAAGACTCCACTCCTGACAAATCC
AGTGACCAGAAATTGAATTAGAAAATTATGATAAACAAAACATGAAAAGGGATAAACAGA
GCTTGAACTCACTGTATGCGTTGGGGCATGAGTTGTGTTAAGATACTTCTCATTGTTGAT
ACTCATACTCATTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACG
CGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGGACTCTAGAGGAT
CACAGGCAGCAACGCTCTGTCATCGTTACAATCAACATGCTACCCTCCGCGAGATCATCC
GTGTTTCAAACCCGGCAGCTTAGTTGCCGTTCTTCCGAATAGCATCGGTAACATGAGCAA
AGTCTGCCGCCTTACAACGGCTCTCCCGCTGACGCCGTCCCGGACTGATGGGCTGCCTGT
ATCGAGTGGTGATTTTGTGCCGAGCTGCCGGTCGGTCGAGTGGTCTGGATTTTGGTTTTA
GGAATTAGAAATTTTATTGATAGAAGTATTTTACAAATACAAATACATACTAAGGGTTTC
TAAAATGCTCAA
   
Sequence Name 01-K117831w-0022-692-A05C-CR3S
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K117831w-0022-692-A05C-CR3S
CATAAATTTCTATTCCTAAAACCAAAATCCAGACCACTCGACCGACCGGCAGCTCGGCAC
AAAATCACCACTCGATACAGGCAGCCCATCAGTCCGGGACGGCGTCAGCGGGAGAGCCGT
TGTAAGGCGGCAGACTTTGCTCATGTTACCGATGCTATTCGGAAGAACGGCAACTAAGCT
GCCGGGTTTGAAACACGGATGATCTCGCGGAGGGTAGCATGTTGATTGTAACGATGACAG
AGCGTTGCTGCCTGTGATCCTCTAGAGTCCATGTGAGCAAAAGGCCAGCAAAAGGCCAGG
AACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCAT
CACAATGAGTATGAGTATCAACAATGAGAAGTATCTTAACACAACTCATGCCCCAACGCA
TACAGTGAGTTCAAGCTCTGTTTATCCCTTTTCATGTTTTGTTTATCATAATTTTCTAAT
TCAATTTCTGGTCACTGGATTTGTCAGGAGTGGAGTCTTGAGCTAGAGGAAGTTTTTGCT
CTTGAAAGAGAAGGAGAGTTTGATAAATATGCTCCCCACAGAGAAAAACTTGGCAATAAA
ATTGCTCCTA
Primers used for PCR 85vr - CR3S, show primer details
   
Sequence Name 29-K117953-0022-692-A05C-85vr
GenBank Accession LN499466 [GenBank]
Sequencing primer gene specific primer, 85vr: 5'-ATGTATTTCTCCCAATGCAACTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K117953-0022-692-A05C-85vr
GGATTTTCTTACAGTGTAGCAGTACTGAGCACTTTTACTGACAAGGTCAGCAAAGTATAT
CCCTTTTCCAAACTGAAACATGGGGTTTAAAAAAAGTTCAGATTCTCTAACAAGTTAACT
AGATAGTGAAGAACTCTCAATTGATAAGATCCCCACAAATAAGACACAGATAGAGTACTG
ACCATGTAACCAGTAGCAGGAGCTTCTGGAGGTGCAATTCTCAGTCCTTGGTTCAATATT
CCAACAAAATTCGTTAATCGAGAACCTGAAAGCAGATTTATTTTGGTTAAACCAAGGGTT
TTGATGATCAACCAGCCAAATATATAGAATCAACCAAACAATGAAATTTCTTGTATCTGT
TTCCCAAAGCATGTAAATGTGGCTGGTTGCAGTTGGCTTACCATGCCATAGGAGCATCTT
ATTGCCAAGTTTTTCTCTGTGGGGAGCATATTTATCAAACTCTCCTTCTCTTTCAAGAGC
AAAAACTTCCTCTAGCTCAAGACTCCACTCCTGACAAATCCAGTGACCAGAAATTGAATT
AGAAAATTATGATAAACAAAACATGAAAAGGGATAAACAGAGCTTGAACTCACTGTATGC
GTTGGGGCATGAGTTGTGTTAAGATACTTCTCATTGTTGATACTCATACTCATTGTGATG
CTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCT
GGCCTTTTGCTGGCCTTTTGCTCACATGGACTCTAGAGGATCACAGGCAGCAACGCTCTG
TCATCGTTACAATCAACATGCTACCCTCCGCGAGATCATCCGTGTTTCAAACCCGGCAGC
TTAGTTGCCGTTCTTCCGAATAGCATCGGTAACATGAGCAAAGTCTGCCGCCTTACAACG
GCTCTCCCGCTGACGCCGTCCCGGACTGATGGGCTGCCTGTATCGAGTGGTGATTTTGTG
CCGAGCTGCCGGGTCGTCGAGTGGTCTGGGATTT
   
Sequence Name 05-K117952-0022-692-A05C-CR3S
GenBank Accession LN488541 [GenBank]
Sequencing primer T-DNA primer, CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117952-0022-692-A05C-CR3S
CATAAATTTCTATTCCTAAAACCAAAATCCAGACCACTCGACCGACCGGCAGCTCGGCAC
AAAATCACCACTCGATACAGGCAGCCCATCAGTCCGGGACGGCGTCAGCGGGAGAGCCGT
TGTAAGGCGGCAGACTTTGCTCATGTTACCGATGCTATTCGGAAGAACGGCAACTAAGCT
GCCGGGTTTGAAACACGGATGATCTCGCGGAGGGTAGCATGTTGATTGTAACGATGACAG
AGCGTTGCTGCCTGTGATCCTCTAGAGTCCATGTGAGCAAAAGGCCAGCAAAAGGCCAGG
AACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCAT
CACAATGAGTATGAGTATCAACAATGAGAAGTATCTTAACACAACTCATGCCCCAACGCA
TACAGTGAGTTCAAGCTCTGTTTATCCCTTTTCATGTTTTGTTTATCATAATTTTCTAAT
TCAATTTCTGGTCACTGGATTTGTCAGGAGTGGAGTCTTGAGCTAGAGGAAGTTTTTGCT
CTTGAAAGAGAAGGAGAGTTTGATAAATATGCTCCCCACAGAGAAAAACTTGGCAATAAG
ATGCTCCTATGGCATGGTAAGCCAACTGCAACCAGCCACATTTACATGCTTTGGGAAACA
GATACAAGAAATTTCATTGTTTGGTTGATTCTATATATTTGGCTGGTTGATCATCAAAAC
CCTTGGTTTAACCAAAATAAATCTGCTTTCAGGTTCTCGATTAACGAATTTTGTTGGAAT
ATTGAACCAAGGACTGAGAATTGCACCTCCAGAAGCTCCTGCTACTGGTTACATGGTCAG
TACTCTATCTGTGTCTTATTTGTGGGGATCTTATCAATTGAGAGTTCTTCACTATCTAGT
TAACTTGTTAGAGAATCTGAACTTTTTTTAAACCCCATGTTTCAGTTTGGAAAAGGGATA
TACTTTGCTGACCTTGTCAGTAAAAGTGCTCAGTACTGCTA

Last Updated on 06.12.2018 16:11