Sequences from confirmation amplicon


Confirmation sequences for line 694D08 and hit At4g02530 are shown.
Line ID 694D08
Locus At4g02530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1114112
Primers used for PCR 33jn - 8474, show primer details
   
Sequence Name 53-K113916-0022-694-D08C-33jn
GenBank Accession LN508219 [GenBank]
Sequencing primer gene specific primer, 33jn: 5'-TCGGAAGGGTAAGAGAGTTTGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K113916-0022-694-D08C-33jn
CACAAAGCGAAACTCTTTCTTTTCGCCTTTTAACAAAGAAATACCGTGGCGCTCTCCACG
CCAAGCGGCTTACGCCTATTTTTGGAGGGCCCTTACTTAATCGTGAGGCCCAACATTTAA
CCTAGTCTGAATGTTTCATTTCGCTAAAAACTTTGTTTGTTGCTACTGGGCCCCAAAGGT
TTGAAAGGCCAGTTCAATTGAAATATCGCATAATCAAATTGAATTTAATCTGCAAGACCA
ATTCGTTAATCAATAAGAGCATACTAGGATTTCAAACCAAATGATTCAGTTCAACGATGT
TTTAAATTATCATCTAGAAATTATAAAATGTGTAATAAAGCATTTTTTTTTTTTTTAAAC
TTGACAAATTCTCTAATTGTAAATTCTAATATAACCTTTAAAATCTAAAAACTAATTTTC
CTTTTTTTTAAAAATTTTTTAAAAATTTTTGAAAAAAAAAATTATTGAAAAAATTTTTGA
AATTGATTGTTTGGAGAAAAATAACACACCCCACCTTTTTAACAAAAAAAAAAAGATTAT
TTCCCATTTTTTTTTCATTTGAAAATGGCTCCATTTCCGGAAAATCTCCTTGGATCACCA
TTGATTATAATGGTCAATTTGGAAAAAAAAAAAAATTATTTCCCATTTTTTTTTC
   
Sequence Name 25-K113915-0022-694-D08C-8409
GenBank Accession LN497535 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K113915-0022-694-D08C-8409
TTCTTTTTCCTTCCAATCACTCACTTCTCAATATTTTTTTTCATTACTTCTTCTTTTTTC
ACAATTCTTTTAAAAATTTTCTCAAAAACAAGCATAATTAGTTTTTAGATTTTAAGGTTT
ATATTAGACTTTACATTTAGAGATTTTTGTCAAGTTTAAAAAAAAAAAAATTGCTTTTTT
CCCCTTTTTTTATTTTCTAAATAATATTTTAAACCTTCTTTAAACTGATTCTTTTGTTTT
GAAACCCTATTTTCCTCTTTTTGATTAACAAATTGTTCTTGCAAATAAATTTCATTTTGA
TTTTCCAATTTTTCATTTGAACTGGCTTTTCAAACCTTTGGCCCCATTACCAACAAACAA
TTTTTTTACCAATTGAAACTTTCAAACTAGTTTAATTTTTGGCCCTCACAATTAATTAAG
GCCCCTCCAAAAATAGGCTTAACCCCTTTGGCGTGAAAACCACCCGTTTTTTTTTATTAA
AAGGCAAAAAAATTTTTCCTTTTTTCCTCCCCACATACCTCCAAATTTTAATTCTAAGGG
AGATTGAATTCTTTTTTTTTAAAATTTTCCCT

Last Updated on 06.12.2018 16:11