Sequences from confirmation amplicon


Confirmation sequences for line 696C04 and hit At1g74700 are shown.
Line ID 696C04
Locus At1g74700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28065770
Primers used for PCR DF12 - 8474, show primer details
   
Sequence Name 27-K029125-022-696-C04C-8409
GenBank Accession LN498249 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K029125-022-696-C04C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTTCAATTTGAGAATGTAACACCAACACAGCA
ACAGAGCAATATGCTAATGATAATAAACACATGGTGGCTCTCAACAAGGGTTTATCAAAA
CAGTAACAGAGCAATAAGCTAATGATAATAAACACATGGTGGCAGTCTCAACAAAGGCTT
ATCAAAATTGTTCTTAAAACAAAAAGTCAAAAGAAGGATCTTTGCAAAATATAATCCGAA
ATTTCAAGTCTTTTTATAGAAGATTCAAGATACTTACGATATGATCCATGTGAGAGTGAG
AGATGAAGAGGAAGTCTTGAGAAATTGCGCGATGTGGGCAACGACCAATGTCGAAAGCTA
TCCGAAGAGATGGAAATATGATGCACGTCTCGTGCCCACCAATCGACAATCCCTCGATCG
GGTAACCTTCAATTTGCATTGCTTTCTTCTTCTCCATCTCACTGTTCCTTAGGGTTTATC
GAATTTTGGCTGTGCTCTTCGTT
   
Sequence Name 28-K029125-022-696-C04C-DF12
GenBank Accession LN498712 [GenBank]
Sequencing primer gene specific primer, DF12: 5'-CAATATTTGAAAACGAAGAGCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K029125-022-696-C04C-DF12
AAGGCCAAAATTCGATAAACCCTAAGGAACAGTGAGATGGAGAAGAAGAAAGCAATGCAA
ATTGAAGGTTACCCGATCGAGGGATTGTCGATTGGTGGGCACGAGACGTGCATCATATTT
CCATCTCTTCGGATAGCTTTCGACATTGGTCGTTGCCCACATCGCGCAATTTCTCAAGAC
TTCCTCTTCATCTCTCACTCTCACATGGATCATATCGTAAGTATCTTGAATCTTCTATAA
AAAGACTTGAAATTTCGGATTATATTTTGCAAAGATCCTTCTTTTGACTTTTTGTTTTAA
GAACAATTTTGATAAGCCTTTGTTGAGACTGCCACCATGTGTTTATTATCATTAGCTTAT
TGCTCTGTTACTGTTTTGATAAACCCTTGTTGAGAGCCACCATGTGTTTATTATCATTAG
CATATTGCTCTGTTGCTGTGTTGGTGTTACATTCTCAAATTGAAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11