Sequences from confirmation amplicon


Confirmation sequences for line 696G09 and hit At2g47210 are shown.
Line ID 696G09
Locus At2g47210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19379525
Primers used for PCR HV47 - 8474, show primer details
   
Sequence Name 73-K101271-0022-696-G09C-8409
GenBank Accession LN512859 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K101271-0022-696-G09C-8409
CCGGAATGAGCCTTTACATTGAATATACCCAATGGTAAAGCTGCAAATCATCTTTCCTAG
CAGAATTTGTGAAGGAAAGCCATTTCCAAGCAACCTGAGAGATACTAAAGACATTTTTCT
TAGCAAATAATACTAACTGTAGAATCATACTACTAACATTAAAATTATCTCAAAACTGAA
AATAGACAGTGGCAACAAATGATTAAAGCCCGCAGCAGCAACAGACCTTCTCATCTGCAG
GAGGACGGCGCTTTAAATCAATTGAAGGCATAAGAGGTGCAACGCCCCCGGTAAGCGCAT
AAACCTGAAATTCATCAACGACGATGAAAATTCAACTACAAAAATATCCAATCGATTCCA
AAGGATTGGTTCGATTCGTTAGAGCTA
   
Sequence Name 73-K101277-0022-696-G09C-HV47
GenBank Accession LN512860 [GenBank]
Sequencing primer gene specific primer, HV47: 5'-TTAGCTCTAACGAATCGAACCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K101277-0022-696-G09C-HV47
TTTGAGTTGATTTTCATCGTCGTTGATGAATTTCAGGTTTATGCGCTTACCGGGGGCGTT
GCACCTCTTATGCCTTCAATTGATTTAAAGCGCCGTCCTCCTGCAGATGAGAAGGTCTGT
TGCTGCTGCGGGCTTTAATCATTTGTTGCCACTGTCTATTTTCAGTTTTGAGATAATTTT
AATGTTAGTAGTATGATTCTACAGTTAGTATTATTTGCTAAGAAAAATGTCTTTAGTATC
TCTCAGGTTGCTTGGAAATGGCTTTCCTTCACAAATTCTGCTAGGAAAGATGATTTGCAG
CTTTACCATTGGGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCACGATTATT

Last Updated on 06.12.2018 16:11