Sequences from confirmation amplicon


Confirmation sequences for line 699B09 and hit At3g61830 are shown.
Line ID 699B09
Locus At3g61830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22888743
Primers used for PCR MN65 - 8474, show primer details
   
Sequence Name 36-K103105-0022-699-B09C-MN65
GenBank Accession LN502184 [GenBank]
Sequencing primer gene specific primer, MN65: 5'-ACAGTTTCACAAAAACACACCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K103105-0022-699-B09C-MN65
TGCTTAACCTCCATTCAAAGCCATGAAGATCTCTAGTCACAAGTTCTTGAGTAGGAGTAG
CTTGTGTCATATCCTAAAAAGTGTAAAATTGGAGTGAGAATTCAAGCTAAAGTTTCTTGA
ATTGAGAAGGTTTCTTTAAGAATATATTTACCAAAGAAGGCAAGCATTCAGTGGCGTGTT
TACGAAGAACAGAGAATCCACCATGAGTGCTTGTATCTGAAGCCGTTAAAATCTTAACAA
ACGAATGAAACTCTTGCTTAGTTGGTCCAACAATAGGTGGATCAAGACTTGTTGGTTCAC
TTTGCTGCAAACAAAACAGTAAATTGAAATCAATTCCAATCAATATAAATTAACACTCAC
TGAAATAAAGTTGAGTTTTTTTTTTAAAAACTTACATCTTCCTCTGGTTGTAATGTGATC
TGAGCGTAAACCTCATCTGTTTCATGCTCCGCCTATAAATCAAAATTTAGTTAACAGATT
CAGGGGAAATAAAACATTTTAACATTTATGATCCAACTTTACCTTTAAAGTGACATCAAG
AACTCGACAAAGTATCTTTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAAATGTCCG
   
Sequence Name 36-K103106-0022-699-B09C-8409
GenBank Accession LN502185 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K103106-0022-699-B09C-8409
GAGCCATTTACAATTGAATATATCCTGCAAAGATACTTTGTCGAGTTCTTGATGTCACTT
TAAAGGTAAAGTTGGATCATAAATGTTAAAATGTTTTATTTCCCCTGAATCTGTTAACTA
AATTTTGATTTATAGGCGGAGCATGAAACAGATGAGGTTTACGCTCAGATCACATTACAA
CCAGAGGAAGATGTAAGTTCTTAAAAAAAAAACTCAACTTTATTTCAGTGAGTGTTAATT
TATATTGATTGGAATTGATTTCAATTTACTGTTTTGTTTGCAGCAAAGTGAACCAACAAG
TCTTGATCCACCTATTGTTGGACCAACTAAGCAAGAGTTTCATTCGTTTGTTAAGATTTT
AACGGCTTCAGATACAAGCACTCATGGTGGATTCTCTGTTCTTCGTAAACACGCCACTGA
ATGCTTGCCTTCTTTGGTAAATATATTCTTAAAGAAACCTTCTCAATTCAAGAAACTTTA
GCTTGAATTCTCACTCCAATTTTACACTTTTTAGGATATGACACAAGCTACTCCTACTCA
AGAACTTGTGACTAGAGATCTTCATGGCTTTGAATGGAGGTTTAAGCATATATTCAGAGG
TATTGATTCTAATCTTGGTGTGTTTTTGGAAAACT

Last Updated on 06.12.2018 16:11