Sequences from confirmation amplicon


Confirmation sequences for line 701C12 and hit At4g01120 are shown.
Line ID 701C12
Locus At4g01120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:482811
Primers used for PCR NE11 - 8474, show primer details
   
Sequence Name 96-K102373-0022-701-C12C-NE11
GenBank Accession LN515385 [GenBank]
Sequencing primer gene specific primer, NE11: 5'-GATTCTCGGTTTGACTGTTTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>96-K102373-0022-701-C12C-NE11
AGATAACACCACTAACACCAATAAACAAACAAAAGGGGATGTCACGAATAAATGAAGTCA
ACGAGAGTGGCACAAGAATACTATACCCATGGCTGTGGCACACCGTTCATGCCAGCAGAG
TTTTGGAAACTCATTGCTGTGGGCATAACAGGAGTCCCCATAGTCACATCGGGTTTCTCA
TTTTCACCTCTAAGAGGCAGACTGTTTTGAGATGAGGGTCTTTCACCTACCAAGTACAAA
AGCCATTAGCTTTAAATAGTTGTAAACATTAAAAGCATACAATTATTTGCGTTGTGTTAT
TTTGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
TCGACGTGTCTACATTCACTCCAAAATGG
Primers used for PCR AJ15 - 3144, show primer details
   
Sequence Name 02-K105456-0022-701-C12C-AJ15
GenBank Accession LN487031 [GenBank]
Sequencing primer gene specific primer, AJ15: 5'-AATTCAGCTGGAAACTCAGATCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K105456-0022-701-C12C-AJ15
TGCAATGTCATAAGCAATAACAAAGTTGGGAGTGCTGAACATAGCAGCAGTGAACATAGG
AGTTCTCAGAGGTTTGTCAAACTAGCCTCAACTCTTTCTTATTCAATAGATACTTAGATA
TATAGAATCTAACGTGGTTTTGATATCAACTAGCTCCGAGAATGATGGCTCTAGCAATGG
TAGTGATGGTAATACAACTGGGGTATGGACTCGTCCTCTCTGCTCTGAACTAGGTTTAGA
TTTATCTTTTGCGATCTTTATTCATATTCTTCTTTTTTTATTACCACAGGGAGACGACAA
TCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGG
GTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCAAACAA
ATCCA
   
Sequence Name 18-K105457-0022-701-C12C-3144
GenBank Accession LN494304 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K105457-0022-701-C12C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTCTCCCTGTGGTAATAAAAAAAGAAGAATATGAATAAAGATCG
CAAAAGATAAATCTAAACCTAGTTCAGAGCAGAGAGGACGAGTCCATACCCCAGTTGTAT
TACCATCACTACCATTGCTAGAGCCATCATTCTCGGAGCTAGTTGATATCAAAACCACGT
TAGATTCTATATATCTAAGTATCTATTGAATAAGAAAGAGTTGAGGCTAGTTTGACAAAC
CTCTGAGAACTCCTATGTTCACTGCTGCTATGTTCAGCACTCCCAACTTTGTTATTGCTT
ATTGACATTGCAAGTCCATCGAACTCTTTCAGCTTTTTCATGAACCCATGATCTGAGTTT

Last Updated on 06.12.2018 16:11