Sequences from confirmation amplicon


Confirmation sequences for line 703E09 and hit At3g01270 are shown.
Line ID 703E09
Locus At3g01270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:83613
Primers used for PCR NL35 - 8474, show primer details
   
Sequence Name 37-K104023-0022-703-E09C-NL35
GenBank Accession LN502593 [GenBank]
Sequencing primer gene specific primer, NL35: 5'-TTCTGAAGCATAATCCCTCTTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K104023-0022-703-E09C-NL35
TTTGATTTGATGCCATATATATATGTGCACACTCAAATCATTTTGGACCTCTCTGTAGTG
TTGTTTGTGAGGAGGAGCGATGAAACGGTTTCCATGGCTGAGAATGGTAGGACCTTGGCT
ACCACCGATCGCGTAAAGCTCCCAATGAGTGTAGTCATTGTTGACAACGTGGACGAAACC
CCATCGGACCCTAGGCATCCTCTGCACAAGTCCTTTTCCGAAATGGTTGTAAGCCACTGT
GACTTGCATCTTCTTGTCATCCATGTTGTTGTTTTGTGCACCAAGCAACATCACCTAAAG
ACAGAACCCAAAAACATAACAAGATCCTTTGGTTAACTTGGGTTGTCATTAATAGTATAA
CGGTCGAGTTAATTACGTTGTGGAATGCTCACATCGTTGTGGTGGGTGAAATGAGAGTTA
GAGATGGTGATTGCGGTGGAACCCATTATAGCGTCGATNAGTCCATCTGGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGC
   
Sequence Name 53-K104024-0022-703-E09C-8409
GenBank Accession LN508136 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K104024-0022-703-E09C-8409
GCCATTTACAGATGGACTTATCGACGCTATAATGGGTTCCACCGCAATCACCATCTCTAA
CTCTCATTTCACCCACCACAACGATGTGAGCATTCCACAACGTAATTAACTCGACCGTTA
TACTATTAATGACAACCCAAGTTAACCAAAGGATCTTGTTATGTTTTTGGGTTCTGTCTT
TAGGTGATGTTGCTTGGTGCACAAAACAACAACATGGATGACAAGAAGATGCAAGTCACA
GTGGCTTACAACCATTTCGGAAAAGGACTTGTGCAGAGGATGCCTAGGGTCCGATGGGGT
TTCGTCCACGTTGTCAACAATGACTACACTCATTGGGAGCTTTACGCGATCGGTGGTAGC
CAAGGTCCTACCATTCTCAGCCATGGAAACCGTTTCATCGCTCCTCCTCACAAACAACAC
TACAGAGAGGTCCAAAATGATTTGAGTGTGCACATATATATNTGGCATCAAATCAAAGTA
CTAATAGAAAACATGTGTATATATNGGTGACAAAGAGGGATTATGC

Last Updated on 06.12.2018 16:11