Sequences from confirmation amplicon


Confirmation sequences for line 705E04 and hit At3g61850 are shown.
Line ID 705E04
Locus At3g61850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22895746
Primers used for PCR NL36 - 8474, show primer details
   
Sequence Name 38-K104023-0022-705-E04C-NL36
GenBank Accession LN502956 [GenBank]
Sequencing primer gene specific primer, NL36: 5'-ATATTTAAAAGAGGGGCCAGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K104023-0022-705-E04C-NL36
CTAGTCAAAACAACAAACCCTCCTTTTGCTTTTTCATATAAATTATTATATTTTATTTTG
CAGGTTTCTTCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCTTGGCTGCTTTCTTTCA
TCATCCATAAAGTGAAAGCTAACGCATAGAGAGAGCCATATCGTCCCAAAAAAAGCAAAA
GTCCAAAAAAAAACAACTCCAAAACATTCTCTCTTAGCTCTTTACTCTTTAGTTTCTCTC
TCTCTCTCTGCCTTTCTCTTTGTTGAAGTTCAGGGATGCTACGAAGTGGACTCAGGTACG
TAAAAAGATATCTCTTTGCTATATCTGTTTGTTTCTAGCTTCTCCCTGACTCTAACGCTC
TCTCTCTCTCTCTCTCTCTCTTTTTATCTCTCTACTCACATAAATATATACGTGTGTGTG
TATGCATGTTTATATGTATGTATAAAACCAGTAGTGGTTATACAGATNGTCTATATAGAG
ATATCAATATGATGTGTTTTAATTTAGACTTTTTATATCTCCAAATAAATGGCTTCATGT
CCGGGAAATCTACGTGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATCTCCGC
   
Sequence Name 54-K104024-0022-705-E04C-8409
GenBank Accession LN508459 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K104024-0022-705-E04C-8409
GCCATTATTCGGATATATAAAAAGTCTAAATTAAAACACATCATATTGATATCTCTATAT
AGACTATCTGTATAACCACTACTGGTTTCATACATACATATAAACATGCATACACACACA
TGTATATATTTATGTGAGTAGAGAGATAAAAAGAGAGAGAGAGAGAGAGAGAGAGCGTAA
GAGTCAGGGAGAAGCTAGAAACAAACAGATATAGCAAAGAGATATCTTTTTACGTACCTG
AGTCCACTTCGTAGCATCCATGAACTTCAACAAAGAGAAAGGCAGAGAGAGAGAGAGAAA
CTAAAGAGTAAAGAGCTAAGAGAGAATGTTTTGGAGTTGTTTTTTTTTGGACTTTTGCTT
TTTTTGGGACGATATGGCTCTCTCTATGCGTTAGCTTTCACTTTATGGATGATGAAAGAA
AGCACCCGAGAGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGAAGACCCCTGCAAAATAA
AATATAATATTTTATATGAAAAAGCAAAGGGAGGGTTTGTTGTTTTTGACTAGTGATAAT
AATGTCTCAGGGCCTCCCTCTCTGGTTCTTTTCGGGCCCCTC

Last Updated on 06.12.2018 16:11