Sequences from confirmation amplicon


Confirmation sequences for line 710G03 and hit At1g49710 are shown.
Line ID 710G03
Locus At1g49710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18392714
Primers used for PCR NB92 - 8474, show primer details
   
Sequence Name 76-K101386-0022-710-G03C-8409
GenBank Accession LN513364 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K101386-0022-710-G03C-8409
GAGCCATTTACAATCACATCAAACTGATCTTTGTCTACCAATGTATAATTTGCCTGATTT
ACTTATGTTACCTTTCCCATCCCGGTTTCGATGACAACCACCATAAGAATCAATCTTAAT
GTTAGTTTTCATCAATGCCTCAAGTGCTTGTAGACGAAAATTCCGAGCACCACAATTAGA
AATAAAAGCAGCTGCAATAGCTCTCTCAGTTTTTGGCTGTACCGGAGACATAATATCATA
CTCCGCCCACGAAAAATATCCAACAGGAACATCTGATGATAGACTAGTGGTCATCACTAT
ATCATAACCTCTCCTGATGAACAA
   
Sequence Name 76-K101392-0022-710-G03C-NB92
GenBank Accession LN513365 [GenBank]
Sequencing primer gene specific primer, NB92: 5'-TTTTCTTGTTCATCAGGAGAGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K101392-0022-710-G03C-NB92
TGTTCCTGTTGGATATTTTTCGTGGGCGGAGTATGATATTATGTCTCCGGTACAGCCAAA
AACTGAGAGAGCTATTGCAGCTGCTTTTATTTCTAATTGTGGTGCTCGGAATTTTCGTCT
ACAAGCACTTGAGGCATTGATGAAAACTAACATTAAGATTGATTCTTATGGTGGTTGTCA
TCGAAACCGGGATGGGAAAGGTAACATAAGTAAATCAGGCAAATTATACATTGGTAGACA
AAGATCAGTTTGATGTGATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
ANAAATGTAGATGTCCGCAGC

Last Updated on 06.12.2018 16:11