Sequences from confirmation amplicon


Confirmation sequences for line 715B09 and hit At5g56270 are shown.
Line ID 715B09
Locus At5g56270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22782781
Primers used for PCR IS06 - 8474, show primer details
   
Sequence Name 25-K114570-0022-715-B09C-IS06
GenBank Accession LN497557 [GenBank]
Sequencing primer gene specific primer, IS06: 5'-AGAAGGTTTGTTTTAGCAATCGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K114570-0022-715-B09C-IS06
CGAGACTCAAAACTTTTTGATTTTACAGGAAACTAGAAGCTTTTGCAGCAGAGATGAGTG
GATCAACAAGAGCCATACGTGAGCCAAGAGTTGTTGTGCAGACAACGAGTGATGTTGACA
TTCTTGATGATGGTTATCGCTGGCGAAAATATGGTCAGAAAGTTGTCAAAGGCAATCCAA
ATCCAAGGTTCTTTATAATCCCCTCCTCTGTTTTGGTCTGGTTCTGTTTATATGTTTATC
GGCAATCACAAGACCAATTCTTTTTTTATAACGTTTTCATAATGTTTTTCTAGGAGTTAT
TACAAATGCACAGCTCCNGGATGTACAGTGAGGAAACATGTTGAAAGAGCTTCTCATGAT
CTCAAATCCGTTATAACAACTTACGAAGGCAAACATAACCATGACGTCCCCGCTGCACGC
AACAGCAGCCACGGAGGCGGTGGTGATAGTGGTAACGGTAACAGCGGCGGTTCAGCCGCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAG
   
Sequence Name 12-K114569-0022-715-B09C-8409
GenBank Accession LN491682 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K114569-0022-715-B09C-8409
CATTTCATTGAATATATCCTGCGGCTGAACCGCCGCTGTTACCGTTACCACTATCACCAC
CGCCTCCGTGGCTGCTGTTGCGTGCAGCGGGGACGTCATGGTTATGTTTGCCTTCGTAAG
TTGTTATAACGGATTTGAGATCATGAGAAGCTCTTTCAACATGTTTCCTCACTGTACATC
CTGGAGCTGTGCATTTGTAATAACTCCTAGAAAAACATTATGAAAACGTTATAAAAAAAG
AATTGGTCTTGTGATTGCCGATAAACATATAAACAGAACCAGACCAAAACAGAGGAGGGG
ATTATAAAGAACCTTGGATTTGGATTGCCTTTGACAACTTTCTGACCATATTTTCGCCAG
CGATAACCATCATCAAGAATGTCAACATCACTCGTTGTCTGCACAACAACTCTTGGCTCA
CGTATGGCTCTTGTTGATCCACTCATCTCTGCTGCAAAAGCTTCTAGTTTCCTGTAAAAT
CAAAAAGTTTTTGAGTCTCGTTTACTGAGTCATCGAGGATTCGGTGTTTCGATTGCTAAA
ACAAAACCTTCT

Last Updated on 06.12.2018 16:11