Sequences from confirmation amplicon


Confirmation sequences for line 715C05 and hit At1g28560 are shown.
Line ID 715C05
Locus At1g28560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10038538
Primers used for PCR MG33 - 8474, show primer details
   
Sequence Name 19-K032923-022-715-C05C-8409
GenBank Accession LN494655 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K032923-022-715-C05C-8409
CATTTACAATTGAATATGATGATGATTAAAGAACCAAAAGAGAAAGAGAGAAGATTTTAC
CTGATGACAATAGACATAACTAGCTCCGACTCTGAATCTTATATCACAGAAGTGTGTACT
CTGCATGTCTGCAGTTCTGTAACGAGGTAAATCCACAGATTTCGCTTCACCTAGAACCAG
TTTCTGCTTTTTCTGTAATTCGCCGGTTAAAACACATTCCCATTTTTTAAGAGCTTCATC
TTTCGAGTTCCAAAGCCAATCTAAAATAGGATAGCTGTAATCTTTAGCCGAGGGATTCCT
TAAATCATTGTGAAAGACATCCTAGACGAGCAAAAAGCATCTGATTAAACCAGCGACGCA
GAAGTTAAACTTACTTAAACATCAAAAGATACAGAAACGAGATGAATTCTTACTTCTATG
AGGAAGTATCCAGAGGGATCATACTTTCCAGCCTTTTGCATCACCTGGTCTGTAGCACAG
TGAATATTGTCCTTCAACTCAGTCAACATTTGACGTCCTAGAACCAAAAATTCTTGGGTC
TGAACAAAAATTTACACAAGTATTGTTAGTATGTACGAGAATTCAGTCAATATTCTATAT
CCCGCATCAAAGATTTACAGAACAATACAGTACCTTAACC
   
Sequence Name 19-K032923-022-715-C05C-MG33
GenBank Accession LN494656 [GenBank]
Sequencing primer gene specific primer, MG33: 5'-ACAGTCGCAAGGTTAAGGTACTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K032923-022-715-C05C-Mg33
TTGACTGAATTCTCGTACTACTAACAATACTTGTGTAAATTTTTGTTCAGACCCAAGAAT
TTTTGGTTCTAGGACGTCAAATGTTGACTGAGTTGAAGGACAATATTCACTGTGCTACAG
ACCAGGTGATGCAAAAGGCTGGAAAGTATGATCCCTCTGGATACTTCCTCATAGAAGTAA
GAATTCATCTCGTTTCTGTATCTTTTGATGTTTAAGTAAGTTTAACTTCTGCGTCGCTGG
TTTAATCAGATGCTTTTTGCTCGTCTAGGATGTCTTTCACAATGATTTAAGGAATCCCTC
GGCTAAAGATTACAGCTATCCTATTTTAGATTGGCTTTGGAACTCGAAAGATGAAGCTCT
TAAAAAATGGGAATGTGTTTTAACCGGCGAATTACAGAAAAAGCAGAAACTGGTTCTAGG
TGAAGCGAAATCTGTGGATTTACCTCGTTACAGAACTGCAGACATGCAGAGTACACACTT
CTGTGATATAAGATTCAGAGTCGGAGCTAGTTATGTCTATTGTCATCAGGTAAAATCTTC
TCTCTTTCTCTTTTGGTTCTTTAATCATCATCATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11