Sequences from confirmation amplicon


Confirmation sequences for line 716H09 and hit At1g60390 are shown.
Line ID 716H09
Locus At1g60390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22248353
Primers used for PCR KH15 - 8474, show primer details
   
Sequence Name 20-K103006-0022-716-H09C-8409
GenBank Accession LN495169 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K103006-0022-716-H09C-8409
GCCATTTACATTGAAGACGGCGTCGTTTTTGCGCGTTATTCTAATAATGTGAGTTCTAAT
GGCAAAACGGTGAATAAGTGGGTTGAGGAGGGTAAATTCTTCAGAGAAGCGATGTTGAAA
GAAGGGACTTTGATGCAAATGCCGGATATTAAAGATAAAATGCCTAAAAGGACATTTTTA
CCCCGAAACATAGTTAAGAATTTACCGTTTTCGTCATCTACGATCGGAGAGATTTGGAGG
GTTTTTGGTGCCGGTGAGAATTCATCAATGGCGGGGATAATCTCGTCGGCGGTTTCAGAG
TGTGAGAGACCGGCGAGTCACGGTGAGACAAAGCGGTGTGTTGGATCAGCGGAGGATATG
ATAGATTTCGCTACGTCGGTGCTCGGACGCGGCGTAGTGGTGAGGACGACGGANAATGTT
GTCGGGTCAAAGAAGAAGGTCGTCATTGGAAAAGTCAATGGAATCAACGGTGGAGATGTA
ACAAGAGCAGTTTCGTGTCACCAGAGTTTGTACCCGTA
   
Sequence Name 68-K103007-0022-716-H09C-KH15
GenBank Accession LN511988 [GenBank]
Sequencing primer gene specific primer, KH15: 5'-AATACGGGTACAAACTCTGGTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K103007-0022-716-H09C-KH15
TTCCTTGACTTTTCCATGACGACCTTCTTCTTTGACCCGACAACATTTTCCGTCGTCCTC
ACCACTACGCCGCGTCCGAGCACCGACGTAGCGAAATCTATCATATCCTCCGCTGATCCA
ACACACCGCTTTGTCTCACCGTGACTCGCCGGTCTCTCACACTCTGAAACCGCCGACGAG
ATTATCCCCGCCATTGATGAATTCTCACCGGCACCAAAAACCCTCCAAATCTCTCCGATC
GTAGATGACGAANACGGTAAATTCTTAACTATGTTTCGGGGTAAAAATGTCCTTTTAGGC
ATTTTATCTTTAATATCCGGCATTTGCATCAAAGTCCCTTCTTTCAACATCGCTTCTCTG
AAGAATTTACCCTCCTCAACCCACTTATTCACCGTTTTGCCATTAGAACTCACATTATTA
GAATAACGCGCAAAAACGACGCCCTCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATNAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATTTCCGCAGCGTTNTNATAAAATGAAAGTACATTTT
GATAAAACGACNAATTACGATCCGTCGTATTTATNGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTNTATNTCGACGTGTCT
Primers used for PCR 12ax - CR3S, show primer details
   
Sequence Name 42-K117832-0022-716-H09C-12ax
GenBank Accession LN504623 [GenBank]
Sequencing primer gene specific primer, 12ax: 5'-AATTCACGAACTACAACACAGACG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K117832-0022-716-H09C-12ax
TTCTACTCGATAAGCCACGGAAGATCACAAACCTTCACTACCTACTCCGAGAACGGCAAC
ACCGGNATATCAATCATTCACAAGCTACAGCAAAAACGGAAACGGAGCTCCGAATGAGTT
CTCGGGTTACGGAACCGGGTCAAACGTAGTTAATACGGGTTTTACCAAATACGGAGAATC
CGCTAACGGAGCTAACGACAGTTTCACGAGCTACGGAGAAAACGGTAATGTTCCGGTTAA
CGAATTTAAAGGATACGGTGACGGAGGTAACGGCGCCGTTTATGGGTTTAAAAATTACAG
AGACCAATCAAACATCGGAGTTGATTCTTTCTCTTCTTACGCTAAAAACTCAAACAACGA
GAAGGTCAATTTCGTAAATTACGGTAAATCTTTTAACCTTGGCTCTGATAATTTCACCGG
TTACGGCCAAGATAACGTCGGAGGTAACGTCAGCTTCAAAACTTACGGCCAAGGACCGAT
TTTGGTTTTAGGAATTAGAAATTTTATTGATAGAAGTATTTTACAAATACAAATACATAC
TAAGGGTTTCTTATATGCTCAA
   
Sequence Name 14-K117831w-0022-716-H09C-CR3S
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, CR3S: 5'-TTGAGCATATAAGAAACCCTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K117831w-0022-716-H09C-CR3S
CATAAATTTCTATTCCTAAAACCAAAATCGGTCCTTGGCCGTAAGTTTTGATGCTGACGT
TACCTCCGACGTTATCTTGGCCGTAACCGGTGAAATTATCAGAGCCAAGGTTAAAAGATT
TACCGTAATTTACGAAATTGACCTTCTCGTTGTTTGAGTTTTTAGCGTAAGAAGAGAAAG
AATCAACTCCGATGTTTGATTGGTCTCTGTAATTTTTAAACCCATAAACGGCGCCGTTAC
CTCCGTCACCGTATCCTTTAAATTCGTTAACCGGAACATTACCGTTTTCTCCGTAGCTCG
TGAAACTGTCGTTAGCTCCGTTAGCGGATTCTCCGTATTTGGTAAAACCCGTATTAACTA
CGTTTGACCCGGTTCCGTAACCCGAGAACTCATTCGGAGCTCCGTTTCCGTTTTTGCTGT
AGCTTGTGAATGATTGATATCCGGTGTTGCCGTTCTCGGAGTAGGTAGTGAAGGTTTGTG
ATCTTCCGTTGGCTTTATCGGAGTAAGAAGTGAATCTTCCGTTTGGTTCGTTTGCGTCTG
TGTTGTATTCCGTGAATT

Last Updated on 06.12.2018 16:11