Sequences from confirmation amplicon


Confirmation sequences for line 717A10 and hit At4g13240 are shown.
Line ID 717A10
Locus At4g13240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7681299
Primers used for PCR DB32 - 8474, show primer details
   
Sequence Name 47-K029916-022-717-A10C-8409
GenBank Accession LN506093 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K029916-022-717-A10C-8409
TAGTTCTGTCAGAATGGCTATCCCTGATAGCTATATCGAGTCTCTTCCAAAGGTGATCTT
CCTTTTTTGTCTTAAAACCGGTATATAGTTTGGATGATTTGCTTGCATTTATTTTAAAAT
ATCATATACTATTTTAAGCAAATCAATAATGGTTATTAAGAAGACTTAGATTATTTTCAG
AATGGAAGAGTAAGCCTTGGGGATTCGTTATACAAGAGCATAACAGAAGAGTGGTTTGAT
CCAGAGCAGTTCTTGTCAACACTTGACTTATCTACAGAGCACAAAGTGTTGGATGTGAAG
AATAGGATTGAGGCCTCAATCGTTATATGGAAAAGAAAACTTCATCTAAAAGACAACAAA
TCTTCTTGGGGATCAGCTGTGAGCTTGGAGAAACGAGAGTTGTTCGAAGAGAGAGCCGAG
ACGATCTTGGTCTTGCTTAAACAAAAGTTCCCTGGTCTTCCCCAATCTTCCCTTGACATT
TCCAAAATTCAGTATAACAAGGTAATGCTCAAATGTTATTGCTTTATTTTGTTATTCCGA
TATGATCGGACAAATCATCATCTAGGAAAGATTCTGAAAATATTG
   
Sequence Name 47-K029916-022-717-A10C-DB32
GenBank Accession LN506094 [GenBank]
Sequencing primer gene specific primer, DB32: 5'-TTCATGACCACCAATATTTTCAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K029916-022-717-A10C-DB32
GATCATATCGGAATAACAAAATAAAGCAATAACATTTGAGCATTACCTTGTTATACTGAA
TTTTGGAAATGTCAAGGGAAGATTGGGGAAGACCAGGGAACTTTTGTTTAAGCAAGACCA
AGATCGTCTCGGCTCTCTCTTCGAACAACTCTCGTTTCTCCAAGCTCACAGCTGATCCCC
AAGAAGATTTGTTGTCTTTTAGATGAAGTTTTCTTTTCCATATAACGATTGAGGCCTCAA
TCCTATTCTTCACATCCAACACTTTGTGCTCTGTAGATAAGTCAAGTGTTGACAAGAACT
GCTCTGGATCAAACCACTCTTCTGTTATGCTCTTGTATAACGAATCCCCAAGGCTTACTC
TTCCATTCTGAAAATAATCTAAGTCTTCTTAATAACCATTATTGATTTGCTTAAAATAGT
ATATGATATTTTAAAATAAATGCAAGCAAATCATCCAAACTATATACCGGTTTTAAGACA
AAAAAGGAAGATCACCTTTGGAAGAGACTCGATATAGCTATCAGGGATAGCCATTTCTGA
CAGAACTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11