Sequences from confirmation amplicon


Confirmation sequences for line 718H12 and hit At1g20925 are shown.
Line ID 718H12
Locus At1g20925 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7291035
Primers used for PCR CV05 - 8474, show primer details
   
Sequence Name 11-K030753-022-718-H12C-8409
GenBank Accession LN490990 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K030753-022-718-H12C-8409
ATTTACAATTGAATATATCCAAAAACGAACCAATGATGAATGTTAGCAAAACATTAAGTG
GCATGAACCACCTGAAAATGACCCCAACAAAATCAACATTTCATTTTCAAGAAGAATCAG
TTCTTTGAATAAAAAACACTTACATTTTCACCATGCTTTCGTATGTAATAGTCTCAGATA
AACTGCTTGCAACAAGAGAAGGACTAAACACATAGAAAACTATCTGTAGAAACCAAAACT
CATAAGAGAATAAATTAACCAAAATTGGTGAATGGCTATGCATGTGACGGAAAACACTGA
CATTGTTGAGTTGTTTTCTTGCATCGTGATTGAGAATGTTAACTTGATCAAGAGCCAAGT
AAAATCCAATTCCTGTTATCAACAGAATCTTCGCAACTGGTATCGATGAGGTTATGAACA
GATCCAAAAGCCTCATCCTCATCTTCGCTTTCAATCTGATACCAAAGAAACCAAAACAAT
ATCTTCTATAGAATAACCAGTTATGCATTCAAATCGAGAAGAATAGAAATACGAAAAACA
TTGAAATCGTGTGAGACGTGATGATGATGACTTACGGAATAGAAAGAACGGAGAATCGAG
AGGAATAGAGAGATCGATCTTCGTATTCTCTGGTTTATCGAGCTAAGAAACAGAGTATCA
ATTATGAAACGTAAAATCCAGAAGATGAGAAACTTGGGAGTGGTAGTTGTGGTTCT
   
Sequence Name 50-K030753-022-718-H12C-CV05
GenBank Accession LN507061 [GenBank]
Sequencing primer gene specific primer, CV05: 5'-CAAAGTATGCGAGAACCACAACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K030753-022-718-H12C-CV05
TTGATACTCTGTTTCTTAGCTCGATAAACCAGAGAATACGAAGATCGATCTCTCTATTCC
TCTCGATTCTCCGTTCTTTCTATTCCGTAAGTCATCATCATCACGTCTCACACGATTTCA
ATGTTTTTCGTATTTCTATTCTTCTCGATTTGAATGCATAACTGGTTATTCTATAGAAGA
TATTGTTTTGGTTTCTTTGGTATCAGATTGAAAGCGAAGATGAGGATGAGGCTTTTGGAT
CTGTTCATAACCTCATCGATACCAGTTGCGAAGATTCTGTTGATAACAGGAATTGGATTT
TACTTGGCTCTTGATCAAGTTAACATTCTCAATCACGATGCAAGAAAACAACTCAACAAT
GTCAGTGTTTTCCGTCACATGCATAGCCATTCACCAATTTTGGTTAATTTATTCTCTTAT
GAGTTTTGGTTTCTACAGATAGTTTTCTATGTGTTTAGTCCTTCTCTTGTTGCAAGCAGT
TTATCTGAGACTATTACATACGAAAGCATGGTGAAAATGTAAGTGTTTTTTATTCAAAGA
ACTGATTCTTCTTGAAAATGAAATGTTGATTTTGTTGGGGTCATTTTCAGGTGGTTCATG
CCACTTAATGTTTTGCTAACATTCATCATTGGTTCGTTTTTGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT

Last Updated on 06.12.2018 16:11