Sequences from confirmation amplicon


Confirmation sequences for line 723E11 and hit At2g02860 are shown.
Line ID 723E11
Locus At2g02860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:832664
Primers used for PCR HM59 - 8474, show primer details
   
Sequence Name 61-K101220-0022-723-E11C-8409
GenBank Accession LN510463 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K101220-0022-723-E11C-8409
GTTAGAAATCTGGTTCTTCGTCGTTCTCTGAATCTGCTTCTCCTTCGAATCATTCTGATT
CGGCTGATGGTGAATCTGTGTCGAAGAATTGTAGTTTAGTGACGTTGGTTCTTAGTTGTA
CAGTTGCCGCTGGAGTTCAATTTGGTTGGGCATTGCAACTTTCTCTTCTTACTCCTTATA
TTCAGGTACTTACTATTACTCTTGTCATTGTTACCATTTCTATCACTTGTGGCTTT
   
Sequence Name 61-K101227-0022-723-E11C-HM59
GenBank Accession LN510464 [GenBank]
Sequencing primer gene specific primer, HM59: 5'-TGCTATAGAGAAAGCCACAAGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K101227-0022-723-E11C-HM59
GAGTATAGTAGTACCTGAATATAGGAGTAAGAAGAGAAAGTTGCAATGCCCAACCAAATT
GAACTCCAGCGGCAACTGTACAACTAAGAACCAACGTCACTAAACTACAATTCTTCGACA
CAGATTCACCATCAGCCGAATCAGAATGATTCGAAGGAGAAGCAGATTCAGAGAACGACG
AAGAACCAGATTCGTTTTGACGATGCTTGGTGACCGTCTCAAGTTCAATTTCCTTCCTCA
AATTCCTATACGGAACCGAGATCGACACCGAGTCACTCATCGTCCTCAACACACAGATCC
CTAATTCGTATCAAAATCAATTTCTCATTCCGATTTAGATTTCTAGGGTTTTGATCTCGT
CGATCTAGATTCGGAGACGATGATCGGAGAGTCAAATTCGGGCGGGTGAATATTTTGATG
AAATCGATTTTTTTGAGGTGATTGAGAGAGAAGACGCGGAAGTGAAGAAGGAAGGAAGAT
TCGTTTTACAGGGAACCGTTGATTTGTAGATTAACGGATGAGATTAAAACCTAACTTAAT
AGTCAACGTTTTCGTCTCGGGCTTTGTGTAATCAGCACCGTTGGATTTGATCGGATCAAA
ACCGTTGCTTCAATTTAAAGGAAGAGCGGACGAAACTCACCGTCACCGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTC

Last Updated on 06.12.2018 16:11