Sequences from confirmation amplicon


Confirmation sequences for line 724B09 and hit At2g44840 are shown.
Line ID 724B09
Locus At2g44840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18495869
Primers used for PCR DN13 - 8474, show primer details
   
Sequence Name 35-K101830-0022-724-B09C-8409
GenBank Accession LN501775 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K101830-0022-724-B09C-8409
TGAGCCTTTACTGGCTAAGCTGAATTTTCCGCATTTGATTGGTTCTTGTAAGTATGAGCC
GGTTAGGATTAGGCCTCGCCGTCGCTCGCCGGAACCGTCAGTCTCCGATCAGTTAACGTC
GGAGCAGAAGAGGGAAAGCCACGTGGATGACGGCGAGTCTAGTTTGGTTGTACCGGAGTT
GGATTTCACGGTGGATCAGTTTTACTTCGATGGTAGTTTATTAATGGACCAATCAGAATG
TTCTTATTCTGATAATCGGATATAATTAGTTTTAAGATTAAGCAAAATTTGTCCAACGAG
TTTTGCTGTATGAAATATCTATCGATGACTCAACAGGTTTTGATCATGATCATATGTAAT
GTGATGGAAATTAAATATTGACGTTTGTTTTTTTCTTCTAAATTCGGTTCCATAGTAAAA
CTTCTATAATAATCTATTTTCTTATATAACTAGGAAAAAAAAGATTTCAAATAGAGCAAG
TAACAATCAATGTGGGATGTATTATATTTCTACTAATCATGGGCTTACTAGTCTATTTAG
AGCATGCCCATTAGTTAAGAATTAAGATAGTAGGATGTATCATGTAGGGATACATAGGAT
TTGTGTTTTTATTATCGTTTTGTCACGGCTATTCTTTTT
   
Sequence Name 79-K101833-0022-724-B09C-DN13
GenBank Accession LN513806 [GenBank]
Sequencing primer gene specific primer, DN13: 5'-GAAAAAGACTAGCCGTGACAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K101833-0022-724-B09C-DN13
TACATGATACATCCTACTATCTTAATTCTTAACTAATGGGCATGCTCTAAATAGACTAGT
AAGCCCATGATTAGTAGAAATATAATACATCCCACATTGATTGTTACTTGCTCTATTTGA
AATCTTTTTTTTCCTAGTTATATAAGAAAATAGATTATTATAGAAGTTTTACTATGGAAC
CGAATTTAGAAGAAAAAAACAAACGTCAATATTTAATTTCCATCACATTACATATGATCA
TGATCAAAACCTGTTGAGTCATCGATAGATATTTCATACAGCAAAACTCGTTGGACAAAT
TTTGCTTAATCTTAAAACTAATTATATCCGATTATCAGAATAAGAACATTCTGATTGGTC
CATTAATAAACTACCATCGAAGTAAAACTGATCCACCGTGAAATCCAACTCCGGTACAAC
CAAACTAGACTCGCCGTCATCCACGTGGCTTTCCCTCTTCTGCTCCGACGTTAACTGATC
GGAGACTGACGGTTCCGGCGAGCGACGGCAAGGCCTAATCCTAACCGGCTCATACTTACA
AGAACCAATCAAATGCGGAAAATTCAGCTTAGCCATGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCANAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11