Sequences from confirmation amplicon


Confirmation sequences for line 728G04 and hit F15O5 are shown.
Line ID 728G04
Locus F15O5 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25998471
Primers used for PCR KN38 - 8474, show primer details
   
Sequence Name 62-K103224-0022-728-G04C-KN38
GenBank Accession LN510730 [GenBank]
Sequencing primer gene specific primer, KN38: 5'-TCAAATTAAAGGAGCTACCAAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K103224-0022-728-G04C-KN38
TTTTAACCTTGAACCAATAAAAAAAGTTCTATTAGTAATATCTATAATTTGTGCATGGTA
GTTGCCAAATTAACATTTCTGCTTGCATGATCAAGTATTAGTAGAGTTTCTATGAAGGCA
ATGGTAAGAACGTTTATATATATTGAGTTAGTGACAAGGACATCCCCATTTCTACATTAT
AGAGGGAAAAAAGCAAGTAACATGAATTGAACAAAATTGATATAGAGAGGGTCGGTACCT
CTTTTTGGCCCATGAGACCCAATATCTCCAAAACGACTGACTAATTAAAATGCAATGCAA
AAGAGAAGAGAAGGAAACAGTTCTAGGAGTGGAAGATAAAGCTACTTAATATGTAGACAT
GCATGCATCTTGCAAGTTGGTATATGCACACAAAAATTACAATTAAAGAGGGAGGAAATT
GATGACATATTGGACTTGTAGCCTCGTGGTTTGTACTAATGATCACCGCTGTGTTATAAT
TCATGTTCAAATCTAGGAGTGGAAGTGTGTGAGTTATCGATGACNAATAAGGAAGACAAC
TCTNATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAA
ATGTAGATTCC
   
Sequence Name 30-K103225-0022-728-G04C-8409
GenBank Accession LN499675 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K103225-0022-728-G04C-8409
TCGGACATGAGCCATTTACAATTGAATATATTAGAGTTGTCTTCCTTATTTGTCATCGAT
AACTCACACACTTCCACTCCTAGATTTGAACATGAATTATAACACAGCGGTGATCATTAG
TACAAACCACGAGGCTACAAGTCCAATATGTCATCAATTTCCTCCCTCTTTAATTGTAAT
TTTTGTGTGCATATACCAACTTGCAAGATGCATGCATGTCTACATATTAAGTAGCTTTAT
CTTCCACTCCTAGAACTGTTTCCTTCTCTTCTCTTTTGCATTGCATTTTAATTAGTCAGT
CGTTTTGGAGATATTGGGTCTCATGGGCCAAAAAGAGGTACCGACCCTCTCTATATCAAT
TTTGTTCAATTCATGTTACTTGCTTTTTTCCCTCTATAATGTAGAAATGGGGATGTCCTT
GTCACTAACTCAATATATATAAACGTTCTTACCATTGCCTTCATAGAAACTCTACTAATA
CTTGATCATGCAAGCAGAAATGTTAATTTGGCAACTACCATGCACAAATTATAGATTTTA
CTAANAGAACTTTTTTTTATTTGGTTCAAGGTTTAAAATAGTTAAAATCCAGTTGTAGCT
NGTTTCCTTGGTAGCTCCTT

Last Updated on 06.12.2018 16:11