Sequences from confirmation amplicon


Confirmation sequences for line 728H06 and hit At3g32050 are shown.
Line ID 728H06
Locus At3g32050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:13068386
Primers used for PCR CR57 - 8474, show primer details
   
Sequence Name 53-K107115-0022-728-H06C-CR57
GenBank Accession LN508172 [GenBank]
Sequencing primer gene specific primer, CR57: 5'-CAAAGCCAACTCCAAAATAAGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K107115-0022-728-H06C-CR57
GCATACATGTGGGTCAATTTCATATGTCCGGAGAAGAGAAGAGATGGTAATTTGTATTAT
TTTTGCATATAATATACTTTCTGTCAAATTCAGTCCAAGTTTTCTATAATGACATATTTG
TGTGTTTCAAAACTGTTTAGCGTGATAAAGTGACAGGAGAACTTCCAGATATGGTTACTT
TTATGGAAGCAACCCATAAGAGAAAGTCTAATGGTATATTTGTGTGCAAAAAGTTGAGTT
GATCGCCAAAAAATATCGAAACATGGAGCACCAAATTCTCAGTCAAAGAGACAATGACGA
TGGTGACACTTTGGATACTAACAATCTTACACAAGAAGAAATTGATGCCATTTACCAAGG
GGTAAAATGCTGAAACTTCTATTATTTTTTAATTATACTTCATGTTTTTTTTTTAACCAT
ACTTTATGTTTTTAATACTTTTTCTTATATGAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAAA
   
Sequence Name 37-K107114-0022-728-H06C-8409
GenBank Accession LN502633 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K107114-0022-728-H06C-8409
GCCATTTACAATTCATATAAGAAAAAGTATTAAAAACATAAAGTATGGTTAAAAAAAAAA
CATGAAGTATAATTAAAAAATAATAGAAGTTTCAGCATTTTACCCCTTGGTAAATGGCAT
CAATTTCTTCTTGTGTAAAATTGTTAGTATCCAAAGTGTCACCATCGTCATTGTCTCTTT
GACTGAAAATTTGGTGCTCCATGTTTCGATATTTTTTGGCGATCAACTCAACTTTTTGCA
CACAAATATACCATTAAACTTTCTCTTATGGGTTGCTTCCATAAAAGTAACCATATCTGG
AAGTTCTCCTGTCACTTTATCACGCTAAACAGTTTTGAAACACACAAATATGTCATTATA
GAAAACTTGGACTGAATTTGACAGAAAGTATATTATATGCAAAAATAATACAAATTACCA
TCTCTTCTCTTCTCCGGACATATGAAATTGACCCACATGTATGCTTAGCTATCCCTAAGC
CACCAAGATCGCTCTTCTTATTTTGGAGTTTGGCTTTG

Last Updated on 06.12.2018 16:11