Sequences from confirmation amplicon


Confirmation sequences for line 729A07 and hit At2g33670 are shown.
Line ID 729A07
Locus At2g33670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14246549
Primers used for PCR RP16 - 8474, show primer details
   
Sequence Name 77-K027490-022-729-A07C-8409
GenBank Accession LN513472 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K027490-022-729-A07C-8409
CTTACATTGAATATATCCTGCCATTTAAATGATCTTTTGATGTATTTTTGGAAATTAAAC
TTCATACCCGGTGCCAAATGGACCTAAGTAACCGAGGATTTTGGAAACATATCTCAAACA
ATGTTTTGGGGAGAAATTAATTAAAGAATGGTTTTGCATAATCTCAAGGAACTTACACTT
ATGAAACCATGACGCATTGTCAAATAATCAGATTTTCTGACAGATCTCAGCATTTGACGA
AAGAAACACATCTACAAAACAGAGTAACAAGGAAAGTCTCTCTTGCTTTAGATACATAGA
AACAAGAGATGGAATAATAAAAACAAACGATCAAAATCTTACAACGTAGAATGAGAATCT
ATTTTTGGCCCAAGGATTGACATGCTCTCTAACAAATGATGTCTCATGTGTGAGCCTAAA
CCTTGAAGGGTCTGTAAATTACACCAAAGTAATAAGAAATTGATATTCTTGTTTGAAATG
TGAATGGCATCTGTAACTATAAGCTCAATAATCTAACCATTCATCATTTCATGATCATTT
ATGACTTCTTCCTCCCAAACTTTCCATCCACGAATCTGCATAATCCAACAATCAAAATCT
CACAAACACATACACATTGCTACGACATTAAGTAAACATTATATATGCAAATATACCTTT
GCTCGTCCAAGCATCATGGTAATAGCACTATAAATGACATGAAACACAG
   
Sequence Name 78-K027490-022-729-A07C-RP16
GenBank Accession LN513617 [GenBank]
Sequencing primer gene specific primer, RP16: 5'-GCATATCTTCATCTTTTTCTTGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K027490-022-729-A07C-RP16
ATGATGCTTGGACGAGCAAAGGTATATTTGCATATATAATGTTTACTTAATGTCGTAGCA
ATGTGTATGTGTTTGTGAGATTTTGATTGTTGGATTATGCAGATTCGTGGATGGAAAGTT
TGGGAGGAAGAAGTCATAAATGATCATGAAATGATGAATGGTTAGATTATTGAGCTTATA
GTTACAGATGCCATTCACATTTCAAACAAGAATATCAATTTCTTATTACTTTGGTGTAAT
TTACAGACCCTTCAAGGTTTAGGCTCACACATGAGACATCATTTGTTAGAGAGCATGTCA
ATCCTTGGGCCAAAAATAGATTCTCATTCTACGTTGTAAGATTTTGATCGTTTGTTTTTA
TTATTCCATCTCTTGTTTCTATGTATCTAAAGCAAGAGAGACTTTCCTTGTTACTCTGTT
TTGTAGATGTGTTTCTTTCGTCAAATGCTGAGATCTGTCAGAAAATCTGATTATTTGACA
ATGCGTCATGGTTTCATAAGTGTAAGTTCCTTGAGATTATGCAAAACCATTCTTTAATTA
ATTTCTCCCCAAAACATTGTTTGAGATATGTTTCCAAAATCCTCGGTTACTTAGGTCCAT
TTGGCACCGGGTATGAAGTTTAATTTCCAAAAATACATCANAAGATCATTGTAAATGGCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCATAAACAAATTATTCTAATT

Last Updated on 06.12.2018 16:11