Sequences from confirmation amplicon


Confirmation sequences for line 734D04 and hit At1g75850 are shown.
Line ID 734D04
Locus At1g75850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28480817
Primers used for PCR JV64 - 8474, show primer details
   
Sequence Name 40-K101326-0022-734-D04C-8409
GenBank Accession LN503649 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K101326-0022-734-D04C-8409
CGGAATGAGCCTTTACATTGAATCATTAACAATTGTCCGGACATGAAGCCATCTTCCTAC
AAAGTTTTCGGATCAATAATTAGTTTAACGCCAGGGCTACACCAGATTCAAGTAATCATA
AAACTTTAAAAATGCATTTTACACCTTTACAATGAAAATCTGAATCTGAACATATAGTGT
AGACAGAGATACAAGCTACAGAGACAATACTGACTAAAAGTTTAGAAAACATATGGCAAC
GAAAGGAAACCATACCAATACTTGATCCACATAGTCAAGCCGATCAGGATGAACTCGGAG
AGTAAAAGTCAAAAGAGAGACAAACAGAGTCATAGCTCCGACAATAGGCATCTCAAGCTG
TGTATCTATCACCTGTAGCCCACACCAAGTCCATAAGTCAAATATATGTATTTCAAGAAA
AATATTGCATTATATGCAGGAGGCTGGGAAAGCAAAGACTCAAAGAATTGAGGCTATTGA
CGAACAGAATCTACAATATGAACTAAGGTGGTTGGATCATCCAGCAATTTGATAGCTTGA
TTTTTAATGGTCTTTCATTTTAAGTTCTACATAATTTTACATCTGCTCCTCTTCTAGCTA
CTGAAGTCTCAAATCTTACAACTAGAGGACAGACATGTTCAAACTACAGTGGCTTATTTT
CCATCATATGTCAAGGAAGGTAGCTGATTTGACGATGCCTAACAAAAGAGCTGATTAGGA
GAAGATGAGCGAAGATTGGAAAAT
   
Sequence Name 40-K101332-0022-734-D04C-JV64
GenBank Accession LN503650 [GenBank]
Sequencing primer gene specific primer, JV64: 5'-TTATTTTCCAATCTTCGCTCATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K101332-0022-734-D04C-JV64
CGTCAATCGCTACCTTCCTTGACATATGATGGAAAATAAGCCACTGTAGTTTGAACATGT
CTGTCCTCTAGTTGTAAGATTTGAGACTTCAGTAGCTAGAAGAGGAGCAGATGTAAAATT
ATGTAGAACTTAAAATGAAAGACCATTAAAAATCAAGCTATCAAATTGCTGGATGATCCA
ACCACCTTAGTTCATATTGTAGATTCTGTTCGTCAATAGCCTCAATTCTTTGAGTCTTTG
CTTTCCCAGCCTCCTGCATATAATGCAATATTTTTCTTGAAATACATATATTTGACTTAT
GGACTTGGTGTGGGCTACAGGTGATAGATACACAGCTTGAGATGCCTATTGTCGGAGCTA
TGACTCTGTTTGTCTCTCTTTTGACTTTTACTCTCCGAGTTCATCCTGATCGGCTTGACT
ATGTGGATCAAGTATTGGTATGGTTTCCTTTCGTTGCCATATGTTTTCTAAACTTTTAGT
CAGTATTGTCTCTGTAGCTTGTATCTCTGTCTACACTATATGTTCAGATTCAGATTTTCA
TTGTAAAGGTGTAAAATGCATTTTTAAAGTTTTATGATTACTTGAATCTGGTGTAGCCCT
GGCGTTAAACTAATTATTGATCCGAAAACTTTGTAGGGTGATGGCTTCATGTCCGGACAA
TTGTTAATTGATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCCATTCAA
AAATGTAGATGTCCGCACC

Last Updated on 06.12.2018 16:11