Sequences from confirmation amplicon


Confirmation sequences for line 741D02 and hit At3g55080 are shown.
Line ID 741D02
Locus At3g55080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20413374
Primers used for PCR PG37 - 8474, show primer details
   
Sequence Name 31-K101744-0022-741-D02C-8409
GenBank Accession LN500100 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K101744-0022-741-D02C-8409
ATTTACATTGAATAAAACACAATGATCCCATTGAAGAACAACACAATGCATAAAACCTCC
CTTATGAGAGATTGTACATGGTTGAGTAACACCAGAAACAGATATAGAGCCAAATGAATA
CTTGTGAAGTAACTTTCTCACCTAAAGCATATGCATACATGAAATCTTCCAAATCTGGGC
GTTCAGTTACTATGGGACAATGTTGTTTGAATGCCTAAGAATCACCAAAAAGTGATTTTA
GAACTCTTATTCACTAAGATGAAAAATAAATTCGACACATGGAAATACATCTACCAAACA
TACACATATGACATATGTATACTACGTACTTGGTTAGCTTGACATAGGACATAATCATAG
TAGCCTTCGTAATATGCTAAGACTTGTTATGAACTGGAGTATAAAACTTGAAGAAGATAA
TTCAAAATTTAAACCGTAAATAATTTTTCAGCGGTATATACACAGGTAATATAGCACCTT
GATAGACCCAACTCGGTTTCTCAATCAAAAGGTCTGATATCACCACGAGACCAAACAGAC
NAGACAAATAAGTTTTGTAACTATNTAGTCTATATGTGTGTGCCTGTCTNATGGTTCCAT
ACA
   
Sequence Name 31-K101750-0022-741-D02C-PG37
GenBank Accession LN500101 [GenBank]
Sequencing primer gene specific primer, PG37: 5'-AGTGTATGAACCCATAAGACAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K101750-0022-741-D02C-PG37
CAAACTTATTTGTCTTGTCTGTTTGGTCTCGTGGTGATATCAGACCTTTTGATTGAGAAA
CCGAGTTGGGTCTATCAAGGTGCTATATTACCTGTGTATATACCGCTGAAAAATTATTTA
CGGTTTAAATTTTGAATTATCTTCTTCAAGTTTTATACTCCAGTTCATAACAAGTCTTAG
CATATTACGAAGGCTACTATGATTATGTCCTATGTCAAGCTAACCAAGTACGTAGTATAC
ATATGTCATATGTGTATGTTTGGTAGATGTATTTCCATGTGTCGAATTTATTTTTCATCT
TAGTGAATAAGAGTTCTAAAATCACTTTTTGGTGATTCTTAGGCATTCAAACAACATTGT
CCCATAGTAACTGAACGCCCAGATTTGGAAGATTTCATGTATGCATATGCTTTAGGTGAG
AAAGTTACTTCACAAGTATTCATTTGGCTCTATATCTGTTTCTGGTGTTACTCAACCATG
TACAATCTCTCATAAGGGAGGTTTTATGCATTGTGTTGTTCTTCAATGGGATCATTGTGT
TGTTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATAT
AGATATCCGC

Last Updated on 06.12.2018 16:11