Sequences from confirmation amplicon


Confirmation sequences for line 746H05 and hit At1g54270 are shown.
Line ID 746H05
Locus At1g54270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20260976
Primers used for PCR GE42 - 8474, show primer details
   
Sequence Name 78-K100941-0022-746-H05C-8409
GenBank Accession LN513652 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K100941-0022-746-H05C-8409
GCCATTTACAATTGAATATATCCTCCAGAGCAGAAAGTGGCGGTTTTTCCAGTACCAGAC
TGTGCCTGCTGGATCACATCAAGACCCTTGCAAAAGGGTACAATTCCTCTTTGCTGAATA
GCAGAAGGCTTTTCGAAACCTAGAAAAAAATCAACCAATCAATCACAGGAAAGAGCACAT
ACGATAAAGTTAATAGGAATCCCAACATAACAAACAAAAGGTTGTTTCTTTAAACCTTCT
TCACTTACCGTAAGCATAGATACCCCTAAGAAGATTCTCTTGCAAACCCATGGCATCAAA
GCTCTCATGGACCTCATCATATGAGGTGAAGAACTCATCCTGTCCATCGAGACTGCACCC
AAACAAACAATAAGATTAGCAAATACAAAAAAAATCTCATCAAAAACGAGTAATGAAACT
AAAGATCTTAGGACTTACACTTCATTCAGCCTCTGGTCAAACTGACGCGTATCAAATTGT
GTTCCTTCCGGTGCGGATCCTGCCATACTACAACAATACACAAAAGAAACAATAGTTTAG
AACCCAGATNGCTNGAGATGATTCTTAAATCTTATAGCCAG
   
Sequence Name 78-K100949-0022-746-H05C-GE42
GenBank Accession LN513653 [GenBank]
Sequencing primer gene specific primer, GE42: 5'-TTGCACAACTCTCTCTGGCTATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K100949-0022-746-H05C-GE42
CTGGGTTCTACTATTGTTTCTTTTGTGTATTGTTGTAGTATGGCAGGATCCGCACCGGAA
GGAACACAATTTGATACGCGTCAGTTTGACCAGAGGCTGAATGAAGTGTAAGTCCTAAGA
TCTTTAGTTTCATTACTCGTTTTTGATGAGATTTTTTTTGTATTTGCTAATCTTATTGTT
TGTTTGGGTGCAGTCTCGATGGACAGGATGAGTTCTTCACCTCATATGATGAGGTCCATG
AGAGCTTTGATGCCATGGGTTTGCAAGAGAATCTTCTTAGGGGTATCTATGCTTACGGTA
AGTGAAGAAGGTTTAAAGAAACAACCTTTTGTTTGTTATGTTGGGATTCCTATTAACTTT
ATCGTATGTGCTCTTTCCTGTGATTGATTGGTTGATTTTTTTCTAGGTTTCGAAAAGCCT
TCTGCTATTCAGCAAAGAGGAATTGTACCCTTTTGCAAGGGTCTTGATGTGATCCAGCAG
GCACAGTCTGGTACTGGAAAAACCGCCACTTTCTGCTCTGGAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT

Last Updated on 06.12.2018 16:11