Sequences from confirmation amplicon


Confirmation sequences for line 751B12 and hit At1g72650 are shown.
Line ID 751B12
Locus At1g72650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27351908
Primers used for PCR BE46 - 8474, show primer details
   
Sequence Name 62-K028750-022-751-B12C-8409
GenBank Accession LN510659 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K028750-022-751-B12C-8409
CCATTAGAAAGGTGATCGGGAGATGACGTAAGAGCTACTAAAATGAAAGATGCTCCATCG
AGTACTGATCATGTGAATGGACATTCAAACGGTGGCAACCACTATTACGCTAGTGAGGAT
TATTCCAGTGAACAGAGGGCTGCAAAACGAGTTAGGAAGCCTACACGAAGATACATTGAA
GAACTTTCGGAAACAGATGATAAACAGCAAAATGACAAGTCAGTGATTCCTTCTAAAGAT
CAGAGGTTGTCCGAAAAATCTGAGGTCAGGTCTATCAGTGTCTCCTCAGGGAAGAGAGTT
ACTGTCACCAGGATGGTATCCCTTGCTGGATCTGAAATTGAGGTTCCTTATGTTTCTCAT
GTCCGGAGAAGCCGTCCAA
   
Sequence Name 62-K028750-022-751-B12C-BE46
GenBank Accession LN510660 [GenBank]
Sequencing primer gene specific primer, BE46: 5'-GAGCCATAATGTTCTCTCTTGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K028750-022-751-B12C-BE46
GAACCTCATTTCAGAATCGCAGGGATACCATCCTGGTGACAGTAACTCTCTTCCCTGAGG
AGACACTGATAGACCTGACCTCAGATTTTTCGGACAACCTCTGATCTTTAGAAGGAATCA
CTGACTTGTCATTTTGCTGTTTATCATCTGTTTCCGAAAGTTCTTCAATGTATCTTCGTG
TAGGCTTCCTAACTCGTTTTGCAGCCCTCTGTTCACTGGAATAATCCTCACTAGCGTAAT
AGTGGTTGCCACCGTTTGAATGTCCATTCACATGATCAGTACTCGATGGAGCATCTTTCA
TTTTAGTAGCTCTTACGTCATCTCCCATCTCCTTTCTAATGGCATTCGTCACATCGTTGG
ACTTCTCTTGATTTCCAATTAACTTGTTATCTTTCACCCTCAAGTTAGTTGTTGGTACAT
CACAAGAATTAATAAGTCCCATTGCAATCCTCCTTTTAAGCCACGTCTTGTCCTTAACAG
TCGTATCAATAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11