Sequences from confirmation amplicon


Confirmation sequences for line 755C03 and hit At3g30721 are shown.
Line ID 755C03
Locus At3g30721 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:12345922
Primers used for PCR 20hj - 8474, show primer details
   
Sequence Name 43-K112479-0022-755-C03C-20hj
GenBank Accession LN504888 [GenBank]
Sequencing primer gene specific primer, 20hj: 5'-CATCATGTTGACTATGTTGATCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K112479-0022-755-C03C-20hj
CTCGATGTTTATCTGCACCGCTGATAGAGGATTTGCTGGATATGTGGATTGAAGGTATAA
AGGTTTATGACTCATTTATGAAAGAGAAATTTACACTAAGAGCTATGTTGTTGTGGAGTA
TCAGTGACTATCCAGGCTTAGGCACATTGTTTGGATGTAAGGTTAAAGGGAAACAAGGCT
GTAACGTTTGTGGAAAGGACACACCTAGTAGATGGTTGAAGTATAGTAAAAAATATGTCT
ATTTGGGCAATAGGAAGCGTCTCAGGAATGGACATCCGTACAGGCGCCAAAAGAGTTGGT
TTGACAACACTATCGAGAATGGGACAGCAAATAGAATTCAAACTAGTGCAGAGATTGCTG
AGATACTTAAAGTTTACAAAAATTACTTTGGAAGACCTTTGGAAAAGGTAACTAAGAGGA
AAAGATCTGAAGTTATTTTAGAGGAAGTCTATGGAGAAGACAGTGATTAATGGAGGTGGA
AGAAATTGTTTTATACTCTTCTTATTACCTTACTGGAAGGTTAGCATTTGGTTTTGTTTA
ATAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCACA
   
Sequence Name 13-K112478-0022-755-C03C-8409
GenBank Accession LN492106 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K112478-0022-755-C03C-8409
TCGGATGAGCATTTCATTGAATATATCCTGCTATTAAACAAAACCAAATGCTAACCTTCC
AGTAAGGTAATAAGAAGAGTATAAAACAATTTCTTCCACCTCCATTAATCACTGTCTTCT
CCATAGACTTCCTCTAAAATAACTTCAGATCTTTTCCTCTTAGTTACCTTTTCCAAAGGT
CTTCCAAAGTAATTTTTGTAAACTTTAAGTATCTCAGCAATCTCTGCACTAGTTTGAATT
CTATTTGCTGTCCCATTCTCGATAGTGTTGTCAAACCAACTCTTTTGGCGCCTGTACGGA
TGTCCATTCCTGAGACGCTTCCTATTGCCCAAATAGACATATTTTTTACTATACTTCAAC
CATCTACTAGGTGTGTCCTTTCCACAAACGTTACAGCCTTGTTTCCCTTTAACCTTACAT
CCAAACAATGTGCCTAAGCCTGGATAGTCACTGATACTCCACAACAACATAGCTCTTAGT
GTAAATTTCTCTTTCATAAATGAGTCATAAACCTTTATACCTTCAATCCACATATCCNGC
AAATCCTCTATCAGCGGTGCTAGATAAACATCGATGTTGTTGCTTGGTGCAGTTGAACCA
GGGATCAACATAGTAAACATGATG

Last Updated on 06.12.2018 16:11