Sequences from confirmation amplicon


Confirmation sequences for line 756G05 and hit At1g63650 are shown.
Line ID 756G05
Locus At1g63650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23600761
Primers used for PCR MH31 - 8474, show primer details
   
Sequence Name 27-K028349-022-756-G05C-8409
GenBank Accession LN498244 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K028349-022-756-G05C-8409
CTTTCATTGAATATATACATAGAAGACATACATAGATAGAAATGCGAAAGCACAACGGAA
ATAAGTTTTTACTTCATTTGCATAATTGCATTATACATTTGCTATTCAAAGAAAGTAGTA
GAAAACTTTTGTTCCCATTAAGATAAAGATTTTGAGATACAAAACATAAAGGGTATTTCC
GTCTTTCTAATGTAGAATCCCCGGAGGAGCGTTATCCAATGGAGAACCAATATGGCTTTG
TAACGCTGAAACCGCCGATAGCAAAGTCTTCACTCGTTCTCTTCTAGCTAAAGTAAGTTT
TTTGCAATTGGAAACGCTAACGCTAGAATTCAAGAACGCTATAACTGAAAACGCTCTTTT
TTTTTTTTTTTTTCGTTGGGGGTTTGGCCCTTAAAAAAGGGCTTCCCTCCAAAAAGGGGT
TTGCTCCCCTTTTCTGGGAGGACCCCTTGAATTGGGCCCCCCCAAACAGGAAACCCTATT
TTCCCTTTTTTTTTCCCACCCCTTTAAAAACTTTTTTAAAAGTCCCCAAAATTTTTTTTT
TTTTTTTTAAAATAAAAAAGGAC
   
Sequence Name 27-K028349-022-756-G05C-MH31
GenBank Accession LN498245 [GenBank]
Sequencing primer gene specific primer, MH31: 5'-TTGTATCACGTTCATGTCCTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K028349-022-756-G05C-mh31
TTGGGACTTTTAGAATGTTTTGATAGTGGTTGGAAAAAAAATAGGAAATATAGGCTTACA
TGTTCGGTCGTGCCAATCTCAAGGACTCCTCCAAGAAACGGGAAGCAAACCACTGTCTGA
AGCGAAGCACTCTTTAAAGAGCAAAACGCCAAACGAAAAAAAAAAAAAAAAAGGGGGTTT
TCGTTTTTGGGGTTTTGGAATTCTGGGGTTGGGTTTCCCATTTGCAAAAAATTTTTTTTA
CTTAAAAAAAAACGGGGGAAAATTTTGTTTTGGGGGGTTTCGGGTTTACAAAGCCTTTTG
GGTTCCCCTTGGGAAAACCCCCCCCGGGGGTTTCTCCTTTAAAAAGAGGGAAATCCCTTT
TTTTTTTTGTTTCCAAAAATTTTTTTTTTAGGGGGAAAAAAATTTTTCTCCTATTTTTTT
TGAAAAGCAAAGGTTTAAGGCATTTTTGCAAATGAAGAAAAAACTTTTTTCCGTGGGGCT
TCCCCATTTCTATCTAGGTGGGCCTCCTAGGAAAATTTCCAATTGAAAAGGGTTTCTTGT
CGGGGAAATCTCCGGGGATCACCATGGGGTTTGAGGGCCAATTGGGAAAAAAAAAAAGAG
TATTTCCCATTTTTTTTTCATTTCAAAAAGGAAAATTCCCCCGGCGTTTTTATAAAAGGA
AGGTCCTTTTTGATAAA

Last Updated on 06.12.2018 16:11