Sequences from confirmation amplicon


Confirmation sequences for line 758C07 and hit At3g57130 are shown.
Line ID 758C07
Locus At3g57130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21150114
Primers used for PCR AC45 - 8474, show primer details
   
Sequence Name 74-K028103-022-758-C07C-8409
GenBank Accession LN512987 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K028103-022-758-C07C-8409
TACATTGAATATATCCTGACAACAATTTAATTAGATATTGAATATGTTTATCTTAATTAA
AGGATGATGGTTATCAAAAAAGGATGATAGGGACTACAAAAAGACCTAGAAATGGTGGTG
GTGGTGATGATACATCGTTGATGGGTCATGATGATGATGATGCAGATTCATTCCTTCCCT
TTGATTGCTATTGTCATCTCCAATATCACGATTTGTAGCACCAAGATTCAGATAAACCAA
TCTTGAATCCAAACTACTTCCGCTCGTGTGTTCGTCTTTCATCCTAGGGTAGATCATTGT
GTTGTTATCGTTACTGTTATTGTTTCCTTCTTCCGTGGATAT
   
Sequence Name 74-K028103-022-758-C07C-AC45
GenBank Accession LN512988 [GenBank]
Sequencing primer gene specific primer, AC45: 5'-GTTATATCACGCGAAGAAGGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K028103-022-758-C07C-AC45
GATCTACCTAGGATGAAGACGAACACACGAGCGGAAGTAGTTTGGATTCAAGATTGGTTT
ATCTGAATCTTGGTGCTACAAATCGTGATATTGGAGATGACAATAGCAATCAAAGGGAAG
GAATGAATCTGCATCATCATCATCATGACCCATCAACGATGTATCATCACCACCACCACC
ATTTCTAGGTCTTTTTGTAGTCCCTATCATCCTTTTTTGATAACCATCATCCTTTAATTA
AGATAAACATATTCAATATCTAATTAAATTGTTGTCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
AAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAA
GCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11