Sequences from confirmation amplicon


Confirmation sequences for line 763F07 and hit At5g43650 are shown.
Line ID 763F07
Locus At5g43650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17533774
Primers used for PCR AC30 - 8474, show primer details
   
Sequence Name 25-K027917-022-763-F07C-8409
GenBank Accession LN497300 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K027917-022-763-F07C-8409
GCCATTTACAATTGAATATTCAATTGTAATGAGACATTGTAGATTTTAAGAAATGTAGAT
TTCACTCATTTCATCATGACCATCCTTTGCTGATTTTTCCTCAATCACTTTAATTCTTCT
CACTAGTTCTTTCTTTAATCTTTGTAATTTCGCAATCTCATCCACGGCTTTTTCGACAAT
CGAATTTTTGTCATTCTGAAATAAAATGAGAATATAAGGAGAAATGATGGTCTTGAAACT
TGTTTCGATATCAATCTATGATGTTCTTTAAACTTGTTTCTATATCAAAATCCTTAAGTA
AAAGAAAATAGTGATAAAAGAAAAGATTCCAGTGCAAAAACTTACAAACATTTTATACAA
ATGATTGTAGTTGGAAATTTATCATACTCAAAACTTTATATTATACCTTACTCAATATAA
ATTTATAGATATTTAAGATAAGCTTCTAAAACCAGATCAATGCATGTAATTAAGCATACA
AACATCAAAGATCAGTGAACAGACATAGTAGTCGAAATTCTGAAAATTTAGTATCCATGA
ATCTAATCCATTTCTACCGAATATCTAATTTTGTGTGC
   
Sequence Name 26-K027917-022-763-F07C-AC30
GenBank Accession LN497774 [GenBank]
Sequencing primer gene specific primer, AC30: 5'-TTTTTACAGTTCAAAATGGCACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K027917-022-763-F07C-AC30
TGGATTAGATTCATGGATACTAAATTTTCAGAATTTCGACTACTATGTCTGTTCACTGAT
CTTTGATGTTTGTATGCTTAATTACATGCATTGATCTGGTTTTAGAAGCTTATCTTAAAT
ATCTATAAATTTATATTGAGTAAGGTATAATATAAAGTTTTGAGTATGATAAATTTCCAA
CTACAATCATTTGTATAAAATGTTTGTAAGTTTTTGCACTGGAATCTTTTCTTTTATCAC
TATTTTCTTTTACTTAAGGATTTTGATATAGAAACAAGTTTAAAGAACATCATAGATTGA
TATCGAAACAAGTTTCAAGACCATCATTTCTCCTTATATTCTCATTTTATTTCAGAATGA
CAAAAATTCGATTGTCGAAAAAGCCGTGGATGAGATTGCGAAATTACAAAGATTAAAGAA
AGAACTAGTGAGAAGAATTAAAGTGATTGAGGAAAAATCAGCAAAGGATGGTCATGATGA
AATGAGTGAAATCTACATTTCTTAAAATCTACAATGTCTCATTACAATTGAATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC

Last Updated on 06.12.2018 16:11