Sequences from confirmation amplicon


Confirmation sequences for line 768C01 and hit At1g07725 are shown.
Line ID 768C01
Locus At1g07725 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2395453
Primers used for PCR DN41 - 8474, show primer details
   
Sequence Name 41-K101830-0022-768-C01C-8409
GenBank Accession LN504025 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K101830-0022-768-C01C-8409
AGCGTCCTAGCGAGAAACTAATGCCCGGGTATACGGGTTTCTATAAGAAACACCCGGTCG
GGTCATGCAATATTGTCAGATTTACCCCTGAAGATCTAAATAATTACATAACCGACCTAT
ATGTTGGTTTAGGAAGATCCGTGCCCGTATCCAAAACTAAGAAGTATATGGTTAGCCCAA
GCCATACGACGGATTTGAAGTAATAGCTCCATGTAAAAAGCCCTCATACGTGTAACTCTG
TGAAATCAACGAATCATAGGCCGGTCCTAGGCTAAAAGCCCATATATCAATTTTGGGCTT
CAAATTATAGACCATAGATATAAAAGTCTACATTTCATTTGCCATATTCAGAATCAGTGT
GTACTTGTGCTTGTATTTGTT
   
Sequence Name 85-K101833-0022-768-C01C-DN41
GenBank Accession LN514527 [GenBank]
Sequencing primer gene specific primer, DN41: 5'-AGAACAAAAGCAAGCACAAGTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K101833-0022-768-C01C-DN41
ACTTTATATCTATGGTCTATAATTTGAAGCCCAAAATTGATATATGGGCTTTTAGCCTAG
GACCGGCCTATGATTCGTTGATTTCACAGAGTTACACGTATGAGGGCTTTTTACATGGAG
CTATTACTTCAAATCCGTCGTATGGCTTGGGCTAACCATATACTTCTTAGTTTTGGATAC
GGGCACGGATCTTCCTAAACCAACATATAGGTCGGTTATGTAATTATTTAGATCTTCAGG
GGTAAATCTGACAATATTGCATGACCCGACCGGGTGTTTCTTATAGAAACCCGTATACCC
GGGCATTAGTTTTCTCGCTATGGACGCTTGGATCTTCATGTTTTCTCATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATA

Last Updated on 06.12.2018 16:11