Sequences from confirmation amplicon


Confirmation sequences for line 771D06 and hit At1g48310 are shown.
Line ID 771D06
Locus At1g48310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17851366
Primers used for PCR NB27 - 8474, show primer details
   
Sequence Name 87-K101222-0022-771-D06C-8409
GenBank Accession LN514735 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K101222-0022-771-D06C-8409
TCGGACATGAGCCATTTACATTGAATATGATGAGAAATCTACATAACGACCTGAAACAAA
GGACTTGAAACAAAGGACTTGTACATAGTAAAACCTCAAATATTTGAGACAGACACTACA
AGCCTTATTTGCAAATTACAGATCACAGAATCTATATATGCCTCTTTCCTGTCAATTCCT
TATATTAGCTTCTCCTCTTAAAATTCAAAAGTCTCAATTTCACATAATTAACAGGTAACC
AACGCGATCATTATAAATCAAGATATTGGGAACAATCAACAAAAATATCAGAACGAAGCA
ATGTCTAGGTAATCACCTTAAAGTCCAATGCCATTAAGAGTTTGTCCAGCTTAGTGACAC
CATCATA
   
Sequence Name 87-K101229-0022-771-D06C-NB27
GenBank Accession LN514736 [GenBank]
Sequencing primer gene specific primer, NB27: 5'-CTATGATGTGGTCACTAAGCTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K101229-0022-771-D06C-NB27
CTTGCTTCGTTCTGATATTTTTGTTGATTGTTCCCATATCTTGATTTATAATGATCGCGT
TGGTTACCTGTTAATTATGTGAAATTGAGACTTTTGAATTTTAAGAGGAGAAGCTAATAT
AAGGAATTGACAGGAAAGAGGCATATATAGATTCTGTGATCTGTAATTTGCAAATAAGGC
TTGTAGTGTCTGTCTCAAATATTTGAGGTTTTACTATGTACAAGTCCTTTGTTTCAAGTC
CTTTGTTTCAGGTCGTTATGTAGATTTCTCATCATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGA

Last Updated on 06.12.2018 16:11