Sequences from confirmation amplicon


Confirmation sequences for line 777F01 and hit At5g17030 are shown.
Line ID 777F01
Locus At5g17030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5603161
Primers used for PCR BG89 - 8474, show primer details
   
Sequence Name 29-K104096-0022-777-F01C-BG89
GenBank Accession LN499259 [GenBank]
Sequencing primer gene specific primer, BG89: 5'-CTCGCTTTATTATCTTCTCCATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K104096-0022-777-F01C-BG89
GCACGATCCTCACGGTTGCTTGGCTTGGATCGAGAAGCGGTCCACTGCTTCTGTAGCGTA
CATTGCCTTTGGTAGAGTCGCGACACCGCCTCCTGTAGAGCTTGTGGCGATAGCACAAGG
ATTGGAATCGAGTAAAGTGCCTTTTGTTTGGTCGCTACAAGAGATGAAAATGACTCATTT
ACCAGAAGGCTTTTTGGATCGGACCAGAGAGCAAGGGATGGTGGTTCCATGGGCACCACA
AGTGGAGCTGCTAAACCATGAAGCAATGGGTGTGTTTGTTTCGCATGGTGGGTGGAACTC
AGTGTTGGAGAGTGTGTCGGCAGGTGTACCGATGATTTGTAGACCGATTTTCGGGGATCA
TGCAATCAATGCAAGATCTGTGGAAGCTGTGTGGGAGATCGGAGTGACGATTAGTAGTGG
AGTCTTCACGAAGGATGGATTTGAGGAGAGTTTGGATCGGGTTTTGGTTCAAGATGATGG
CAAGAAGATGAAGGTTAATGCTAAAAAGCTTGAAGAACTAGCACAAGAAGCTGTCTCTAC
CAAAGGAAGCTCCTTTGAGAATTTTGGAGGATTGTTGGACGAAGTTGTGAACTTTGGATA
AACATGTAAACAACACCTACTATGCTATCTCCGGACAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCC
   
Sequence Name 45-K104097-0022-777-F01C-8409
GenBank Accession LN505512 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K104097-0022-777-F01C-8409
TAGTAGGTGTTGTTTACATGTTTATCCAAAGTTCACAACTTCGTCCAACAATCCTCCAAA
ATTCTCAAAGGAGCTTCCTTTGGTAGAGACAGCTTCTTGTGCTAGTTCTTCAAGCTTTTT
AGCATTAACCTTCATCTTCTTGCCATCATCTTGAACCAAAACCCGATCCAAACTCTCCTC
AAATCCATCCTTCGTGAAGACTCCACTACTAATCGTCACTCCGATCTCCCACACAGCTTC
CACAGATCTTGCATTGATTGCATGATCCCCGAAAATCGGTCTACAAATCATCGGTACACC
TGCCGACACACTCTCCAACACTGAGTTCCACCCACCATGCGAAACAAACACACCCATTGC
TTCATGGTTTAGCAGCTCCACTTGTGGTGCCCATGGAACCACCATCCCTTGCTCTCTGGT
CCGATCCAAAAAGCCTTCTGGTAAATGAGTCATTTTCATCTCTTGTAGCGACCAAACAAA
AGGCACTTTACTCGATTCCAATCCTTGTGCTATCGCCACAAGCTCTACAGGAGGCGGTGT
CGCGACTCTACCAAAGGCAATGTACGCTACAGAAGCAGTGGACCGCTTCTCGATCCAAGC
CAAGCAACCGTGAGGATCGTGCACTAGCGTTGATGTTTGCGATGGAGAA
Primers used for PCR 61wl - 8474, show primer details
   
Sequence Name 76-K118414-0022-777-F01C-61wl
GenBank Accession LN513462 [GenBank]
Sequencing primer gene specific primer, 61wl: 5'-TTGAAAACAGGAAAGAGTTCAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K118414-0022-777-F01C-61wl
CGTATCCGAAGCTTCTCAATTCGATTTTGAGGATTGGAAAAGCTAAGAAAACAGAGATCT
TTTTGGGGATAAATTAGTGTCTTGTTTGGTTAATTAATTGGGTTTAATGTAGATTTAAAC
ATTAAGGACCTTCTCTAGTTATCTCATATGGTTGGCTCCTTTTTGCTTTGACCTTTTTTT
GGGGGTTTGTTTCTATTACTCAGTGATTCGTGTTTTAGGTGCAAGATATTTTTTTAGACT
CTATCTTTGTCACCCCCTAGGGGGTTTAAAATTGTAAGATTGACTTGTGGTGATGTTTCT
CATTAAAATCGTCCACTTTAAAGTGTGCTTTGACAATTTTATCAAACAAAACTTTGAATT
TAGTCATCATTATTTTACTCTTATTTGTTACCCCATATGAAATAAAACTACCACTAAATT
AAATAAATACAACTATTTAATTTATGAAAACGAAAATGCCATCTAAATTAAATTATTGAC
ACGTAACAATAAATATTAAGATAGCATAAAACGTTTATTTGTACTTCGAAAACTTATACG
TCAATAATTTATTATTCCCGCCCACATGTCCGGGAAAATTGTAAATACTACCAAACATTC
CCAGGATACATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCG
   
Sequence Name 28-K118413-0022-777-F01C-8409
GenBank Accession LN499047 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118413-0022-777-F01C-8409
CCATTTACAATTGAATGTATCCTGGGAATGTTTGGTAGTATTTACAATTTTCCCGGACAT
GTGGGCGGGAATAATAAATTATTGACGTATAAGTTTTCGAAGTACAAATAAACGTTTTAT
GCTATCTTAATATTTATTGTTACGTGTCAATAATTTAATTTAGATGGCATTTTCGTTTTC
ATAAATTAAATAGTTGTATTTATTTAATTTAGTGGTAGTTTTATTTCATATGGGGTAACA
AATAAGAGTAAAATAATGATGACTAAATTCAAAGTTTTGTTTGATAAAATTGTCAAAGCA
CACTTTAAAGTGGACGATTTTAATGAGAAACATCACCACAAGTCAATCTTACAATTTTAA
ACCCCCTAGGGGGTGACAAAGATAGAGTCTAAAAAAATATCTTGCACCTAAAACACGAAT
CACTGAGTAATAGAAACAAACCCCCAAAAAAAGGTCAAAGCAAAAAGGAGCCAACCATAT
GAGATAACTAGAGAAGGTCCCTAATGTTTAAATCTACATTAAACCCAATTAATTAACCAA
ACAAGACACTAATTTATCCCCAAAAAGATCTCTGTTTTCTTAGCTTTTCCAATCCTCAAA
ATCGAATTGAGAAGCTTCGGAATACGGTAGTAGAGATACGGTCGGGGAAATTAATCTCTC
CTTGAACTCTTTCGGGGTTTTCAA

Last Updated on 06.12.2018 16:11